{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T02:11:28Z","timestamp":1773627088224,"version":"3.50.1"},"reference-count":0,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[1987,1,1]],"date-time":"1987-01-01T00:00:00Z","timestamp":536457600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[1987,1]]},"abstract":"<jats:p>\n            The catabolism of propane by \u201c\n            <jats:italic>Nocardia paraffinicum<\/jats:italic>\n            \u201d (\n            <jats:italic>Rhodococcus rhodochrous<\/jats:italic>\n            ) has been shown to involve CO\n            <jats:sub>2<\/jats:sub>\n            fixation after its oxidation to propionic acid. \u201c\n            <jats:italic>N. paraffinicum<\/jats:italic>\n            \u201d failed to grow on either propane or 1-propanol in the absence of CO\n            <jats:sub>2<\/jats:sub>\n            . The rate of propane utilization was directly related to the initial CO\n            <jats:sub>2<\/jats:sub>\n            concentration, and Warburg respirometry suggested that CO\n            <jats:sub>2<\/jats:sub>\n            was required for the catabolism of 1-propanol, propionaldehyde, and propionate but not for 2-propanol. These data also suggested that the predominant pathway for the utilization of propane by \u201c\n            <jats:italic>N. paraffinicum<\/jats:italic>\n            \u201d was through 1-propanol. The use of [2-\n            <jats:sup>14<\/jats:sup>\n            C]propane and\n            <jats:sup>14<\/jats:sup>\n            CO\n            <jats:sub>2<\/jats:sub>\n            confirmed the catabolism of propane and the fixation of CO\n            <jats:sub>2<\/jats:sub>\n            . Through the use of these isotopes and the pyruvate carboxylase inhibitor sodium arsenite, the labeled 2,4-dinitrophenylhydrazine derivative of pyruvate was trapped and isolated via thin-layer chromatography. The trapping of [\n            <jats:sup>14<\/jats:sup>\n            C]pyruvate in this manner was considered to be indicative of the presence of the methylmalonyl coenzyme A pathway for CO\n            <jats:sub>2<\/jats:sub>\n            fixation.\n          <\/jats:p>","DOI":"10.1128\/aem.53.1.65-69.1987","type":"journal-article","created":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T03:58:37Z","timestamp":1577851117000},"page":"65-69","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":13,"title":["Role of Carbon Dioxide in Catabolism of Propane by \u201c\n            <i>Nocardia paraffinicum<\/i>\n            \u201d (\n            <i>Rhodococcus rhodochrous<\/i>\n            )"],"prefix":"10.1128","volume":"53","author":[{"given":"Gregory J.","family":"MacMichael","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, Mississippi State University, Mississippi State, Mississippi 39762"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lewis R.","family":"Brown","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Mississippi State University, Mississippi State, Mississippi 39762"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.53.1.65-69.1987","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.53.1.65-69.1987","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T20:39:29Z","timestamp":1645648769000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/aem.53.1.65-69.1987"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1987,1]]}},"alternative-id":["10.1128\/aem.53.1.65-69.1987"],"URL":"https:\/\/doi.org\/10.1128\/aem.53.1.65-69.1987","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[1987,1]]},"assertion":[{"value":"1987-01-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}