{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T15:12:32Z","timestamp":1791904352952,"version":"4.3.4"},"reference-count":0,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[1987,4,1]],"date-time":"1987-04-01T00:00:00Z","timestamp":544233600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[1987,4]]},"abstract":"<jats:p>\n            An antiserum raised against whole cells of\n            <jats:italic>Thiobacillus ferrooxidans<\/jats:italic>\n            was allowed to react with a variety of acidophilic and nonacidophilic bacteria in an enzyme-linked immunosorbent assay and an indirect immunofluorescence assay. Both experiments demonstrated that the antiserum was specific at the species level. This preparation was used to evaluate the role of\n            <jats:italic>T. ferrooxidans<\/jats:italic>\n            in the microbial desulfurization process. Leaching experiments were performed, and the numbers of\n            <jats:italic>T. ferrooxidans<\/jats:italic>\n            cells and other bacteria were estimated by using a combined immunofluorescence-DNA-fluorescence staining technique that was adapted for this purpose. Nonsterile coal samples inoculated with\n            <jats:italic>T. ferrooxidans<\/jats:italic>\n            yielded high concentrations of soluble iron after 16 days. After this period, however,\n            <jats:italic>T. ferrooxidans<\/jats:italic>\n            cells could no longer be detected by the immunofluorescence assay, whereas the DNA-fluorescence staining procedure demonstrated a large number of microorganisms on the coal particles. These results indicate that\n            <jats:italic>T. ferrooxidans<\/jats:italic>\n            is removed by competition with different acidophilic microorganisms that were originally present on the coal.\n          <\/jats:p>","DOI":"10.1128\/aem.53.4.660-664.1987","type":"journal-article","created":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T04:01:13Z","timestamp":1577851273000},"page":"660-664","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":56,"title":["A Combined Immunofluorescence-DNA-Fluorescence Staining Technique for Enumeration of\n            <i>Thiobacillus ferrooxidans<\/i>\n            in a Population of Acidophilic Bacteria"],"prefix":"10.1128","volume":"53","author":[{"given":"Gerard","family":"Muyzer","sequence":"first","affiliation":[{"name":"Department of Biochemistry, University of Leiden, 2333 AL Leiden,  and Department of Microbiology and Enzymology, Delft University of Technology, 2628 BC Delft, 2 The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anke C.","family":"de Bruyn","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Leiden, 2333 AL Leiden,  and Department of Microbiology and Enzymology, Delft University of Technology, 2628 BC Delft, 2 The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diederik J. M.","family":"Schmedding","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Leiden, 2333 AL Leiden,  and Department of Microbiology and Enzymology, Delft University of Technology, 2628 BC Delft, 2 The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Piet","family":"Bos","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Leiden, 2333 AL Leiden,  and Department of Microbiology and Enzymology, Delft University of Technology, 2628 BC Delft, 2 The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Westbroek","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Leiden, 2333 AL Leiden,  and Department of Microbiology and Enzymology, Delft University of Technology, 2628 BC Delft, 2 The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gijs J.","family":"Kuenen","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Leiden, 2333 AL Leiden,  and Department of Microbiology and Enzymology, Delft University of Technology, 2628 BC Delft, 2 The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.53.4.660-664.1987","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.53.4.660-664.1987","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T20:40:45Z","timestamp":1645648845000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/aem.53.4.660-664.1987"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,4]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1987,4]]}},"alternative-id":["10.1128\/aem.53.4.660-664.1987"],"URL":"https:\/\/doi.org\/10.1128\/aem.53.4.660-664.1987","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[1987,4]]},"assertion":[{"value":"1987-04-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}