{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T06:14:41Z","timestamp":1762409681029},"reference-count":0,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[1990,6,1]],"date-time":"1990-06-01T00:00:00Z","timestamp":644198400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[1990,6]]},"abstract":"<jats:p>Experimental bioreactors operated as recirculated closed systems were inoculated with bacterial cultures that utilized methane, propane, and tryptone-yeast extract as aerobic carbon and energy sources and degraded trichloroethylene (TCE). Up to 95% removal of TCE was observed after 5 days of incubation. Uninoculated bioreactors inhibited with 0.5% Formalin and 0.2% sodium azide retained greater than 95% of their TCE after 20 days. Each bioreactor consisted of an expanded-bed column through which the liquid phase was recirculated and a gas recharge column which allowed direct headspace sampling. Pulses of TCE (20 mg\/liter) were added to bioreactors, and gas chromatography was used to monitor TCE, propane, methane, and carbon dioxide. Pulsed feeding of methane and propane with air resulted in 1 mol of TCE degraded per 55 mol of substrate utilized. Perturbation studies revealed that pH shifts from 7.2 to 7.5 decreased TCE degradation by 85%. The bioreactors recovered to baseline activities within 1 day after the pH returned to neutrality.<\/jats:p>","DOI":"10.1128\/aem.56.6.1702-1709.1990","type":"journal-article","created":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T04:25:07Z","timestamp":1577852707000},"page":"1702-1709","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":74,"title":["Biodegradation of Trichloroethylene in Continuous-Recycle Expanded-Bed Bioreactors"],"prefix":"10.1128","volume":"56","author":[{"given":"T. J.","family":"Phelps","sequence":"first","affiliation":[{"name":"Institute for Applied Microbiology, University of Tennessee, Knoxville, Tennessee 37932-2567, and Environmental Sciences Division, Oak Ridge National Laboratory, P. O. Box 2008, Oak Ridge, Tennessee 37831-60382"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. J.","family":"Niedzielski","sequence":"additional","affiliation":[{"name":"Institute for Applied Microbiology, University of Tennessee, Knoxville, Tennessee 37932-2567, and Environmental Sciences Division, Oak Ridge National Laboratory, P. O. Box 2008, Oak Ridge, Tennessee 37831-60382"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. M.","family":"Schram","sequence":"additional","affiliation":[{"name":"Institute for Applied Microbiology, University of Tennessee, Knoxville, Tennessee 37932-2567, and Environmental Sciences Division, Oak Ridge National Laboratory, P. O. Box 2008, Oak Ridge, Tennessee 37831-60382"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. E.","family":"Herbes","sequence":"additional","affiliation":[{"name":"Institute for Applied Microbiology, University of Tennessee, Knoxville, Tennessee 37932-2567, and Environmental Sciences Division, Oak Ridge National Laboratory, P. O. Box 2008, Oak Ridge, Tennessee 37831-60382"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. C.","family":"White","sequence":"additional","affiliation":[{"name":"Institute for Applied Microbiology, University of Tennessee, Knoxville, Tennessee 37932-2567, and Environmental Sciences Division, Oak Ridge National Laboratory, P. O. Box 2008, Oak Ridge, Tennessee 37831-60382"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.56.6.1702-1709.1990","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.56.6.1702-1709.1990","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T20:49:14Z","timestamp":1645649354000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/aem.56.6.1702-1709.1990"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,6]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1990,6]]}},"alternative-id":["10.1128\/aem.56.6.1702-1709.1990"],"URL":"https:\/\/doi.org\/10.1128\/aem.56.6.1702-1709.1990","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,6]]},"assertion":[{"value":"1990-06-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}