{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T12:40:43Z","timestamp":1773664843282,"version":"3.50.1"},"reference-count":0,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[1990,7,1]],"date-time":"1990-07-01T00:00:00Z","timestamp":646790400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[1990,7]]},"abstract":"<jats:p>To investigate the microbiology and epidemiology of the 1,700 sporadic cases of listeriosis that occur annually in the United States, we developed a multilocus enzyme electrophoresis (MEE) typing system for Listeria monocytogenes. We studied 390 isolates by MEE. Eighty-two electrophoretic types (ETs) were defined. Two distinct clusters of ETs, ET group A (ETGA) and ET group B (ETGB), separated at a genetic distance of 0.440, were identified. Strains of ETGB were associated with perinatal listeriosis (P = 0.03). All strains of H antigen type a were in ETGA, while all strains of H antigen type b were in ETGB. Among 328 clinical isolates from cases of literiosis, 55 ETs of L. monocytogenes were defined. Thirty-four ETs were identified among 62 isolates from food products. The mean number of strains per ET (5.2) was significantly higher among clinical isolates than among food-borne isolates. Examination of isolates from outbreaks further documented the link between cases and contaminated food products. In one investigation, we found 11 different ETs, ruling out a single common source as a cause of that outbreak. By examining a large number of isolates collected over a specified time in diverse geographic locations in the United States, we have begun to establish a baseline for the study of the epidemiology of listeriosis by MEE.<\/jats:p>","DOI":"10.1128\/aem.56.7.2133-2141.1990","type":"journal-article","created":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T04:26:01Z","timestamp":1577852761000},"page":"2133-2141","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":132,"title":["Analysis of clinical and food-borne isolates of Listeria monocytogenes in the United States by multilocus enzyme electrophoresis and application of the method to epidemiologic investigations"],"prefix":"10.1128","volume":"56","author":[{"given":"W F","family":"Bibb","sequence":"first","affiliation":[{"name":"Meningitis and Special Pathogens Branch, Centers for Disease Control, Atlanta, Georgia 30333."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B G","family":"Gellin","sequence":"additional","affiliation":[{"name":"Meningitis and Special Pathogens Branch, Centers for Disease Control, Atlanta, Georgia 30333."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"Weaver","sequence":"additional","affiliation":[{"name":"Meningitis and Special Pathogens Branch, Centers for Disease Control, Atlanta, Georgia 30333."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B","family":"Schwartz","sequence":"additional","affiliation":[{"name":"Meningitis and Special Pathogens Branch, Centers for Disease Control, Atlanta, Georgia 30333."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B D","family":"Plikaytis","sequence":"additional","affiliation":[{"name":"Meningitis and Special Pathogens Branch, Centers for Disease Control, Atlanta, Georgia 30333."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M W","family":"Reeves","sequence":"additional","affiliation":[{"name":"Meningitis and Special Pathogens Branch, Centers for Disease Control, Atlanta, Georgia 30333."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R W","family":"Pinner","sequence":"additional","affiliation":[{"name":"Meningitis and Special Pathogens Branch, Centers for Disease Control, Atlanta, Georgia 30333."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C V","family":"Broome","sequence":"additional","affiliation":[{"name":"Meningitis and Special Pathogens Branch, Centers for Disease Control, Atlanta, Georgia 30333."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.56.7.2133-2141.1990","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.56.7.2133-2141.1990","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T20:48:49Z","timestamp":1645649329000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/aem.56.7.2133-2141.1990"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,7]]},"references-count":0,"journal-issue":{"issue":"7","published-print":{"date-parts":[[1990,7]]}},"alternative-id":["10.1128\/aem.56.7.2133-2141.1990"],"URL":"https:\/\/doi.org\/10.1128\/aem.56.7.2133-2141.1990","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,7]]},"assertion":[{"value":"1990-07-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}