{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T12:32:05Z","timestamp":1791894725939,"version":"4.3.4"},"reference-count":0,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[1992,1,1]],"date-time":"1992-01-01T00:00:00Z","timestamp":694224000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[1992,1]]},"abstract":"<jats:p>\n            Copper-resistant strains of\n            <jats:italic>Pseudomonas syringae<\/jats:italic>\n            carrying the\n            <jats:italic>cop<\/jats:italic>\n            operon produce periplasmic copper-binding proteins, and this sequestration outside the cytoplasm has been proposed as a resistance mechanism. In this study, strain PS61 of\n            <jats:italic>P. syringae<\/jats:italic>\n            carrying the cloned\n            <jats:italic>cop<\/jats:italic>\n            operon accumulated more total cellular copper than without the operon. Several other copper-resistant pseudomonads with homology to\n            <jats:italic>cop<\/jats:italic>\n            were isolated from plants, and these bacteria also accumulated copper. Two highly resistant species accumulated up to 115 to 120 mg of copper per g (dry weight) of cells.\n            <jats:italic>P. putida<\/jats:italic>\n            08891 was more resistant to several metals than\n            <jats:italic>P. syringae<\/jats:italic>\n            pv. tomato PT23, but this increased resistance was not correlated with an increased accumulation of metals other than copper. Several metals were accumulated by both PT23 and\n            <jats:italic>P. putida<\/jats:italic>\n            , but when copper was added to induce the\n            <jats:italic>cop<\/jats:italic>\n            operon, there was generally no increase of accumulation of the other metals, suggesting that the\n            <jats:italic>cop<\/jats:italic>\n            operon does not contribute to accumulation of these other metals. The exceptions were aluminum for PT23 and iron for\n            <jats:italic>P. putida<\/jats:italic>\n            , which accumulated to higher levels when copper was added to the cultures. The results of this study support the role of copper sequestration in the copper resistance mechanism of\n            <jats:italic>P. syringae<\/jats:italic>\n            and suggest that this mechanism is common to several copper-resistant\n            <jats:italic>Pseudomonas<\/jats:italic>\n            species found on plants to which antimicrobial copper compounds are applied for plant disease control.\n          <\/jats:p>","DOI":"10.1128\/aem.58.1.274-278.1992","type":"journal-article","created":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T04:36:11Z","timestamp":1577853371000},"page":"274-278","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":53,"title":["Accumulation of Copper and Other Metals by Copper-Resistant Plant-Pathogenic and Saprophytic Pseudomonads"],"prefix":"10.1128","volume":"58","author":[{"given":"Donald A.","family":"Cooksey","sequence":"first","affiliation":[{"name":"Department of Plant Pathology, University of California, Riverside, California 92521-0122"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hamid R.","family":"Azad","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology, University of California, Riverside, California 92521-0122"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.58.1.274-278.1992","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.58.1.274-278.1992","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T20:53:12Z","timestamp":1645649592000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/aem.58.1.274-278.1992"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1992,1]]}},"alternative-id":["10.1128\/aem.58.1.274-278.1992"],"URL":"https:\/\/doi.org\/10.1128\/aem.58.1.274-278.1992","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,1]]},"assertion":[{"value":"1992-01-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}