{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T07:08:21Z","timestamp":1791356901431,"version":"4.3.1"},"reference-count":0,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[1992,6,1]],"date-time":"1992-06-01T00:00:00Z","timestamp":707356800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[1992,6]]},"abstract":"<jats:p>Reductive dechlorination of tetrachloroethene (perchloroethylene; PCE) was observed at 20 degrees C in a fixed-bed column, filled with a mixture (3:1) of anaerobic sediment from the Rhine river and anaerobic granular sludge. In the presence of lactate (1 mM) as an electron donor, 9 microM PCE was dechlorinated to ethene. Ethene was further reduced to ethane. Mass balances demonstrated an almost complete conversion (95 to 98%), with no chlorinated compounds remaining (less than 0.5 micrograms\/liter). When the temperature was lowered to 10 degrees C, an adaptation of 2 weeks was necessary to obtain the same performance as at 20 degrees C. Dechlorination by column material to ethene, followed by a slow ethane production, could also be achieved in batch cultures. Ethane was not formed in the presence of bromoethanesulfonic acid, an inhibitor of methanogenesis. The high dechlorination rate (3.7 mumol.l-1.h-1), even at low temperatures and considerable PCE concentrations, together with the absence of chlorinated end products, makes reductive dechlorination an attractive method for removal of PCE in bioremediation processes.<\/jats:p>","DOI":"10.1128\/aem.58.6.1996-2000.1992","type":"journal-article","created":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T04:41:15Z","timestamp":1577853675000},"page":"1996-2000","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":249,"title":["Complete biological reductive transformation of tetrachloroethene to ethane"],"prefix":"10.1128","volume":"58","author":[{"given":"W P","family":"de Bruin","sequence":"first","affiliation":[{"name":"Department of Microbiology, Wageningen Agricultural University, The Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M J","family":"Kotterman","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Wageningen Agricultural University, The Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M A","family":"Posthumus","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Wageningen Agricultural University, The Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G","family":"Schraa","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Wageningen Agricultural University, The Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A J","family":"Zehnder","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Wageningen Agricultural University, The Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.58.6.1996-2000.1992","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.58.6.1996-2000.1992","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T05:02:28Z","timestamp":1645592548000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/aem.58.6.1996-2000.1992"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,6]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1992,6]]}},"alternative-id":["10.1128\/aem.58.6.1996-2000.1992"],"URL":"https:\/\/doi.org\/10.1128\/aem.58.6.1996-2000.1992","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,6]]},"assertion":[{"value":"1992-06-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}