{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T06:20:49Z","timestamp":1790317249155,"version":"4.1.0"},"reference-count":0,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[1993,4,1]],"date-time":"1993-04-01T00:00:00Z","timestamp":733622400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[1993,4]]},"abstract":"<jats:p>\n            A methanotroph (strain 68-1), originally isolated from a trichloroethylene (TCE)-contaminated aquifer, was identified as the type I methanotroph\n            <jats:italic>Methylomonas methanica<\/jats:italic>\n            on the basis of intracytoplasmic membrane ultrastructure, phospholipid fatty acid profile, and 16S rRNA signature probe hybridization. Strain 68-1 was found to oxidize naphthalene and TCE via a soluble methane monooxygenase (sMMO) and thus becomes the first type I methanotroph known to be able to produce this enzyme. The specific whole-cell sMMO activity of 68-1, as measured by the naphthalene oxidation assay and by TCE biodegradation, was comparatively higher than sMMO activity levels in\n            <jats:italic>Methylosinus trichosporium<\/jats:italic>\n            OB3b grown in the same copper-free conditions. The maximal naphthalene oxidation rates of\n            <jats:italic>Methylomonas methanica<\/jats:italic>\n            68-1 and\n            <jats:italic>Methylosinus trichosporium<\/jats:italic>\n            OB3b were 551 \u00b1 27 and 321 \u00b1 16 nmol h\n            <jats:sup>-1<\/jats:sup>\n            mg of protein\n            <jats:sup>-1<\/jats:sup>\n            , respectively. The maximal TCE degradation rates of\n            <jats:italic>Methylomonas methanica<\/jats:italic>\n            68-1 and\n            <jats:italic>Methylosinus trichosporium<\/jats:italic>\n            OB3b were 2,325 \u00b1 260 and 995 \u00b1 160 nmol h\n            <jats:sup>-1<\/jats:sup>\n            mg of protein\n            <jats:sup>-1<\/jats:sup>\n            , respectively. The substrate affinity of 68-1 sMMO to naphthalene (\n            <jats:italic>\n              K\n              <jats:sub>m<\/jats:sub>\n            <\/jats:italic>\n            , 70 \u00b1 4 \u03bcM) and TCE (\n            <jats:italic>\n              K\n              <jats:sub>m<\/jats:sub>\n            <\/jats:italic>\n            , 225 \u00b1 13 \u03bcM), however, was comparatively lower than that of the sMMO of OB3b, which had affinities of 40 \u00b1 3 and 126 \u00b1 8 \u03bcM, respectively. Genomic DNA slot and Southern blot analyses with an sMMO gene probe from\n            <jats:italic>Methylosinus trichosporium<\/jats:italic>\n            OB3b showed that the sMMO genes of 68-1 have little genetic homology to those of OB3b. This result may indicate the evolutionary diversification of the sMMOs.\n          <\/jats:p>","DOI":"10.1128\/aem.59.4.960-967.1993","type":"journal-article","created":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T04:47:19Z","timestamp":1577854039000},"page":"960-967","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":119,"title":["Soluble Methane Monooxygenase Production and Trichloroethylene Degradation by a Type I Methanotroph,\n            <i>Methylomonas methanica<\/i>\n            68-1"],"prefix":"10.1128","volume":"59","author":[{"given":"Sung-Cheol","family":"Koh","sequence":"first","affiliation":[{"name":"Center for Environmental Biotechnology, The University of Tennessee, Knoxville, Tennessee 37932-2567"},{"name":"Graduate Program in Ecology, The University of Tennessee, Knoxville, Tennessee 37932-2567"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John P.","family":"Bowman","sequence":"additional","affiliation":[{"name":"Center for Environmental Biotechnology, The University of Tennessee, Knoxville, Tennessee 37932-2567"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary S.","family":"Sayler","sequence":"additional","affiliation":[{"name":"Center for Environmental Biotechnology, The University of Tennessee, Knoxville, Tennessee 37932-2567"},{"name":"Graduate Program in Ecology, The University of Tennessee, Knoxville, Tennessee 37932-2567"},{"name":"Department of Microbiology, The University of Tennessee, Knoxville, Tennessee 37932-2567"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.59.4.960-967.1993","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.59.4.960-967.1993","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T20:55:08Z","timestamp":1645649708000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/aem.59.4.960-967.1993"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,4]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1993,4]]}},"alternative-id":["10.1128\/aem.59.4.960-967.1993"],"URL":"https:\/\/doi.org\/10.1128\/aem.59.4.960-967.1993","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,4]]},"assertion":[{"value":"1993-04-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}