{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T00:14:21Z","timestamp":1759104861066},"reference-count":0,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[1994,10,1]],"date-time":"1994-10-01T00:00:00Z","timestamp":780969600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[1994,10]]},"abstract":"<jats:p>Strains of Listeria monocytogenes serotype 4b account for a large fraction of sporadic listeriosis cases, as well as all major food-borne epidemics attributed to this pathogen. We have identified a set of three monoclonal antibodies which showed a high degree of specificity for strains of L. monocytogenes serotype 4b. Two of these antibodies (c74.33 and c74.180, isotypes immunoglobulin M [IgM] and IgG3, respectively) recognized all serotype 4b strains, whereas antibody c74.22 (isotype IgG1) failed to recognize certain epidemic-associated strains. The corresponding antigens were located on the surface of the bacteria and were expressed following bacterial growth in different media and over a wide range of temperatures (4, 22, and 37 degrees C). Heating L. monocytogenes cells at 80,90, or 100 degrees C abolished reactivity for c74.22 but not for c74.33 MAb. These MAbs were negative for all of the non-Listeria strains tested, including representatives of several gram-negative and gram-positive species. The surface antigen recognized by c74.22 appeared to be associated with the ability of the bacteria to enter (invade) mammalian cells in culture.<\/jats:p>","DOI":"10.1128\/aem.60.10.3548-3552.1994","type":"journal-article","created":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T04:53:03Z","timestamp":1577854383000},"page":"3548-3552","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":34,"title":["Monoclonal antibodies with a high degree of specificity for Listeria monocytogenes serotype 4b"],"prefix":"10.1128","volume":"60","author":[{"given":"S","family":"Kathariou","sequence":"first","affiliation":[{"name":"Department of Microbiology, University of Hawaii, Honolulu 96822."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C","family":"Mizumoto","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Hawaii, Honolulu 96822."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R D","family":"Allen","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Hawaii, Honolulu 96822."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A K","family":"Fok","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Hawaii, Honolulu 96822."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A A","family":"Benedict","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Hawaii, Honolulu 96822."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.60.10.3548-3552.1994","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.60.10.3548-3552.1994","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T20:57:38Z","timestamp":1645649858000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/aem.60.10.3548-3552.1994"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,10]]},"references-count":0,"journal-issue":{"issue":"10","published-print":{"date-parts":[[1994,10]]}},"alternative-id":["10.1128\/aem.60.10.3548-3552.1994"],"URL":"https:\/\/doi.org\/10.1128\/aem.60.10.3548-3552.1994","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,10]]},"assertion":[{"value":"1994-10-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}