{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T07:24:15Z","timestamp":1791271455044,"version":"4.1.0"},"reference-count":0,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[1994,2,1]],"date-time":"1994-02-01T00:00:00Z","timestamp":760060800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[1994,2]]},"abstract":"\n Two\n Rhodococcus<\/jats:italic>\n strains which were isolated from a trichloroethylene (TCE)-degrading bacterial mixture and\n Rhodococcus rhodochrous<\/jats:italic>\n ATCC 21197 mineralized vinyl chloride (VC) and TCE. Greater than 99.9% of a 1-mg\/liter concentration of VC was degraded by cell suspensions. [1,2-\n 14<\/jats:sup>\n C]VC was degraded by cell suspensions, with the production of greater than 66%\n 14<\/jats:sup>\n CO\n 2<\/jats:sub>\n and 20%\n 14<\/jats:sup>\n C-aqueous phase products and incorporation of 10% of the\n 14<\/jats:sup>\n C into the biomass. Cultures that utilized propane as a substrate were able to mineralize greater than 28% of [1,2-\n 14<\/jats:sup>\n C]TCE to\n 14<\/jats:sup>\n CO\n 2<\/jats:sub>\n , with approximately 40% appearing in\n 14<\/jats:sup>\n C-aqueous phase products and another 10% of\n 14<\/jats:sup>\n C incorporated into the biomass. VC degradation was oxygen dependent and occurred at a pH range of 5 to 10 and temperatures of 4 to 35\u00b0C. Cell suspensions degraded up to 5 mg of TCE per liter and up to 40 mg of VC per liter. Propane competitively inhibited TCE degradation. Resting cell suspensions also degraded other chlorinated aliphatic hydrocarbons, such as chloroform, 1,1-dichloroethylene, and 1,1,1-trichloroethane. The isolates degraded a mixture of aromatic and chlorinated aliphatic solvents and utilized benzene, toluene, sodium benzoate, naphthalene, biphenyl, and\n n<\/jats:italic>\n -alkanes ranging in size from propane to hexadecane as carbon and energy sources. The environmental isolates appeared more catabolically versatile than\n R. rhodochrous<\/jats:italic>\n ATCC 21197. The data report that environmental isolates of\n Rhodococcus<\/jats:italic>\n species and\n R. rhodochrous<\/jats:italic>\n ATCC 21197 have the potential to degrade TCE and VC in addition to a variety of aromatic and chlorinated aliphatic compounds either individually or in mixtures.\n <\/jats:p>","DOI":"10.1128\/aem.60.2.542-548.1994","type":"journal-article","created":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T04:56:01Z","timestamp":1577854561000},"page":"542-548","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":101,"title":["Aerobic Mineralization of Trichloroethylene, Vinyl Chloride, and Aromatic Compounds by\n Rhodococcus<\/i>\n Species"],"prefix":"10.1128","volume":"60","author":[{"given":"K. J.","family":"Malachowsky","sequence":"first","affiliation":[{"name":"Center for Environmental Biotechnology, University of Tennessee, Knoxville, Tennessee 37932-2567"},{"name":"Department of Microbiology, University of Tennessee, Knoxville, Tennessee 37996"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. J.","family":"Phelps","sequence":"additional","affiliation":[{"name":"Center for Environmental Biotechnology, University of Tennessee, Knoxville, Tennessee 37932-2567"},{"name":"Environmental Sciences Division, Oak Ridge National Laboratories, Oak Ridge, Tennessee 37831"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. B.","family":"Teboli","sequence":"additional","affiliation":[{"name":"Department of Organic Chemistry, The University, Newcastle upon Tyne NEI 7RU, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. E.","family":"Minnikin","sequence":"additional","affiliation":[{"name":"Department of Organic Chemistry, The University, Newcastle upon Tyne NEI 7RU, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. C.","family":"White","sequence":"additional","affiliation":[{"name":"Center for Environmental Biotechnology, University of Tennessee, Knoxville, Tennessee 37932-2567"},{"name":"Environmental Sciences Division, Oak Ridge National Laboratories, Oak Ridge, Tennessee 37831"},{"name":"Department of Microbiology, University of Tennessee, Knoxville, Tennessee 37996"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.60.2.542-548.1994","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.60.2.542-548.1994","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T20:59:48Z","timestamp":1645649988000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/aem.60.2.542-548.1994"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,2]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1994,2]]}},"alternative-id":["10.1128\/aem.60.2.542-548.1994"],"URL":"https:\/\/doi.org\/10.1128\/aem.60.2.542-548.1994","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,2]]},"assertion":[{"value":"1994-02-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}