{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T06:03:38Z","timestamp":1791266618033,"version":"4.1.0"},"reference-count":62,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[1998,1,1]],"date-time":"1998-01-01T00:00:00Z","timestamp":883612800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[1998,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Trichloroethylene (TCE) was removed from soils by using a wheat rhizosphere established by coating seeds with a recombinant, TCE-degrading\n            <jats:italic>Pseudomonas fluorescens<\/jats:italic>\n            strain that expresses the\n            <jats:italic>tomA<\/jats:italic>\n            <jats:sup>+<\/jats:sup>\n            (toluene\n            <jats:italic>o<\/jats:italic>\n            -monooxygenase) genes from\n            <jats:italic>Burkholderia cepacia<\/jats:italic>\n            PR1\n            <jats:sub>23<\/jats:sub>\n            (TOM\n            <jats:sub>23C<\/jats:sub>\n            ). A transposon integration vector was used to insert\n            <jats:italic>tomA<\/jats:italic>\n            <jats:sup>+<\/jats:sup>\n            into the chromosome of\n            <jats:italic>P. fluorescens<\/jats:italic>\n            2-79, producing a stable strain that expressed constitutively the monooxygenase at a level of 1.1 nmol\/min \u00b7 mg of protein (initial TCE concentration, 10 \u03bcM, assuming that all of the TCE was in the liquid) for more than 280 cell generations (36 days). We also constructed a salicylate-inducible\n            <jats:italic>P. fluorescens<\/jats:italic>\n            strain that degraded TCE at an initial rate of 2.6 nmol\/min \u00b7 mg of protein in the presence of 10 \u03bcM TCE [cf.\n            <jats:italic>B. cepacia<\/jats:italic>\n            G4 PR1\n            <jats:sub>23<\/jats:sub>\n            (TOM\n            <jats:sub>23C<\/jats:sub>\n            ), which degraded TCE at an initial rate of 2.5 nmol\/min \u00b7 mg of protein]. A constitutive strain,\n            <jats:italic>P. fluorescens<\/jats:italic>\n            2-79TOM, grew (maximum specific growth rate, 0.78 h\n            <jats:sup>\u22121<\/jats:sup>\n            ) and colonized wheat (3 \u00d7 10\n            <jats:sup>6<\/jats:sup>\n            CFU\/cm of root) as well as wild-type\n            <jats:italic>P. fluorescens<\/jats:italic>\n            2-79 (maximum specific growth rate, 0.77 h\n            <jats:sup>\u22121<\/jats:sup>\n            ; level of colonization, 4 \u00d7 10\n            <jats:sup>6<\/jats:sup>\n            CFU\/cm of root). Rhizoremediation of TCE was demonstrated by using microcosms containing the constitutive monooxygenase-expressing microorganism, soil, and wheat. These closed microcosms degraded an average of 63% of the initial TCE in 4 days (20.6 nmol of TCE\/day \u00b7 plant), compared to the 9% of the initial TCE removed by negative controls consisting of microcosms containing wild-type\n            <jats:italic>P. fluorescens<\/jats:italic>\n            2-79-inoculated wheat, uninoculated wheat, or sterile soil.\n          <\/jats:p>","DOI":"10.1128\/aem.64.1.112-118.1998","type":"journal-article","created":{"date-parts":[[2019,12,19]],"date-time":"2019-12-19T18:53:06Z","timestamp":1576781586000},"page":"112-118","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":130,"title":["Rhizoremediation of Trichloroethylene by a Recombinant, Root-Colonizing\n            <i>Pseudomonas fluorescens<\/i>\n            Strain Expressing Toluene\n            <i>ortho<\/i>\n            -Monooxygenase Constitutively"],"prefix":"10.1128","volume":"64","author":[{"given":"Dennis C.","family":"Yee","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Chemical and Biochemical Engineering, University of California, Irvine, Irvine, California 92697-2575"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer A.","family":"Maynard","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Chemical and Biochemical Engineering, University of California, Irvine, Irvine, California 92697-2575"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas K.","family":"Wood","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Chemical and Biochemical Engineering, University of California, Irvine, Irvine, California 92697-2575"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1021\/es00049a001"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/etc.5620141206"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0045-6535(90)90100-8"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-6-735"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.61.5.1946-1952.1995"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0038-0717(89)90039-4"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.3109\/10408448909037475"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1094\/Phyto-81-954"},{"key":"e_1_3_2_11_2","first-page":"376","article-title":"XL1-Blue: a high efficiency plasmid transforming recA Escherichia coli strain with beta-galactosidase selection.","volume":"5","author":"Bullock W. O.","year":"1987","unstructured":"Bullock W. O. Fernandez J. M. Short J. M. XL1-Blue: a high efficiency plasmid transforming recA Escherichia coli strain with beta-galactosidase selection. BioTechniques 5 1987 376 379","journal-title":"BioTechniques"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(94)00844-I"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6941.1996.tb00307.x"},{"key":"e_1_3_2_14_2","doi-asserted-by":"crossref","unstructured":"Curl\nE. A.\nTruelove\nB.\nThe rhizosphere.\n1986\nSpringer-Verlag\nBerlin Germany","DOI":"10.1007\/978-3-642-70722-3"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(93)90533-9"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(93)90344-3"},{"key":"e_1_3_2_17_2","unstructured":"Environmental Health Center. 1997. Trichloroethylene (C 2 HCl 3 ) chemical backgrounder. 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