{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T06:20:47Z","timestamp":1790317247140,"version":"4.1.0"},"reference-count":39,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[1998,1,1]],"date-time":"1998-01-01T00:00:00Z","timestamp":883612800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[1998,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The influence of trichloroethylene (TCE) on a mixed culture of four different toluene-degrading bacterial strains (\n            <jats:italic>Pseudomonas putida<\/jats:italic>\n            mt-2,\n            <jats:italic>P. putida<\/jats:italic>\n            F1,\n            <jats:italic>P. putida<\/jats:italic>\n            GJ31, and\n            <jats:italic>Burkholderia cepacia<\/jats:italic>\n            G4) was studied with a fed-batch culture. The strains were competing for toluene, which was added at a very low rate (31 nmol mg of cells [dry weight]\n            <jats:sup>\u22121<\/jats:sup>\n            h\n            <jats:sup>\u22121<\/jats:sup>\n            ). All four strains were maintained in the mixed culture at comparable numbers when TCE was absent. After the start of the addition of TCE, the viabilities of\n            <jats:italic>B. cepacia<\/jats:italic>\n            G4 and\n            <jats:italic>P. putida<\/jats:italic>\n            F1 and GJ31 decreased 50- to 1,000-fold in 1 month. These bacteria can degrade TCE, although at considerably different rates.\n            <jats:italic>P. putida<\/jats:italic>\n            mt-2, which did not degrade TCE, became the dominant organism. Kinetic analysis showed that the presence of TCE caused up to a ninefold reduction in the affinity for toluene of the three disappearing strains, indicating that inhibition of toluene degradation by TCE occurred. While\n            <jats:italic>P. putida<\/jats:italic>\n            mt-2 took over the culture, mutants of this strain which could no longer grow on\n            <jats:italic>p<\/jats:italic>\n            -xylene arose. Most of them had less or no\n            <jats:italic>meta<\/jats:italic>\n            -cleavage activity and were able to grow on toluene with a higher growth rate. The results indicate that cometabolic degradation of TCE has a negative effect on the maintenance and competitive behavior of toluene-utilizing organisms that transform TCE.\n          <\/jats:p>","DOI":"10.1128\/aem.64.1.208-215.1998","type":"journal-article","created":{"date-parts":[[2019,12,19]],"date-time":"2019-12-19T18:53:28Z","timestamp":1576781608000},"page":"208-215","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":28,"title":["Effect of Trichloroethylene on the Competitive Behavior of Toluene-Degrading Bacteria"],"prefix":"10.1128","volume":"64","author":[{"given":"Astrid E.","family":"Mars","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Biochemistry, University of Groningen, 9747 AG Groningen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gjalt T.","family":"Prins","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Biochemistry, University of Groningen, 9747 AG Groningen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pieter","family":"Wietzes","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Biochemistry, University of Groningen, 9747 AG Groningen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wim","family":"de Koning","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Biochemistry, University of Groningen, 9747 AG Groningen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dick B.","family":"Janssen","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Biochemistry, University of Groningen, 9747 AG Groningen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2911(08)60119-8"},{"key":"e_1_3_2_3_2","unstructured":"Bailey\nJ. 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