{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T14:58:54Z","timestamp":1791298734447,"version":"4.1.0"},"reference-count":17,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[1998,1,1]],"date-time":"1998-01-01T00:00:00Z","timestamp":883612800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[1998,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Two\n            <jats:italic>Acanthamoeba<\/jats:italic>\n            species, fed at three temperatures, expelled vesicles containing living\n            <jats:italic>Legionella pneumophila<\/jats:italic>\n            cells. Vesicles ranged from 2.1 to 6.4 \u03bcm in diameter and theoretically could contain several hundred bacteria. Viable\n            <jats:italic>L. pneumophila<\/jats:italic>\n            cells were observed within vesicles which had been exposed to two cooling tower biocides for 24 h. Clusters of bacteria in vesicles were not dispersed by freeze-thawing and sonication. Such vesicles may be agents for the transmission of legionellosis associated with cooling towers, and the risk may be underestimated by plate count methods.\n          <\/jats:p>","DOI":"10.1128\/aem.64.1.279-286.1998","type":"journal-article","created":{"date-parts":[[2019,12,19]],"date-time":"2019-12-19T18:53:40Z","timestamp":1576781620000},"page":"279-286","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":198,"title":["Production of Respirable Vesicles Containing Live\n            <i>Legionella pneumophila<\/i>\n            Cells by Two\n            <i>Acanthamoeba<\/i>\n            spp"],"prefix":"10.1128","volume":"64","author":[{"given":"Sharon G.","family":"Berk","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Center for the Management, Utilization and Protection of Water Resources, Tennessee Technological University, Cookeville, Tennessee 38505,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca S.","family":"Ting","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Center for the Management, Utilization and Protection of Water Resources, Tennessee Technological University, Cookeville, Tennessee 38505,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Glenn W.","family":"Turner","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Biology Department, Middle Tennessee State University, Murfreesboro, Tennessee 371322"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca J.","family":"Ashburn","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Center for the Management, Utilization and Protection of Water Resources, Tennessee Technological University, Cookeville, Tennessee 38505,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-140-6-1253"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.64.2.668-673.1996"},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","unstructured":"Bratbak\nG.\nMicroscope methods for measuring bacterial biovolume: epifluorescence microscopy scanning electron microscopy and transmission electron microscopy\nHandbook of methods in aquatic microbial ecology.\nKemp\nP. F.\nSherr\nB. F.\nSherr\nE. B.\nCole\nJ. J.\n1993\n309\n317\nLewis Publishers\nBoca Raton Fla","DOI":"10.1201\/9780203752746-37"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.62.8.3254-3261.1994"},{"key":"e_1_3_2_6_2","unstructured":"Fields\nB. S. Legionella and protozoa: interaction of a pathogen and its natural host\nLegionella: current status and emerging perspectives.\nBarbaree\nJ. M.\nBreiman\nR. F.\nDufour\nA. P.\n1993\n129\n136\nAmerican Society for Microbiology\nWashington D.C"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.47.3.467-471.1984"},{"key":"e_1_3_2_8_2","unstructured":"Lilly\nJ. A.\nBerk\nS. G.\nUse of rRNA-targeted oligonucleotide probes for detection of Legionella in amoebae and vesicles released by amoebae abstr. D-109\nAbstracts of the 96th General Meeting of the American Society for Microbiology 1996.\n1996\n261\nAmerican Society for Microbiology\nWashington D.C"},{"key":"e_1_3_2_9_2","unstructured":"Newsome\nA. L.\nSeeman\nC.\nTing\nR. S.\nWilson\nG. A.\nBerk\nS. G.\nAcid phosphatase localization in Acanthamoeba feeding on Legionella and Escherichia coli abstr. N-167\nAbstracts of the 96th General Meeting of the American Society for Microbiology 1996.\n1996\n351\nAmerican Society for Microbiology\nWashington D.C"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(93)91877-O"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.33.12.1179"},{"key":"e_1_3_2_12_2","first-page":"678","article-title":"Current views on the relationships between amoebae, Legionellae and man.","volume":"22","author":"Rowbotham T. J.","year":"1986","unstructured":"Rowbotham T. J. Current views on the relationships between amoebae, Legionellae and man. Israel J. Med. Sci. 22 1986 678 689","journal-title":"Israel J. Med. Sci."},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-444601-4.50015-1"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.59.10.3245-3249.1993"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00182412"},{"key":"e_1_3_2_16_2","unstructured":"Sutherland\nE. E.\nM.S. thesis.\n1993\nTennessee Technological University\nCookeville"},{"key":"e_1_3_2_17_2","unstructured":"Sutherland\nE. E.\nBerk\nS. G.\nBiocide-induced encystment of protozoa from cooling towers abstr. Q-14\nAbstracts of the 92nd General Meeting of the American Society for Microbiology 1992.\n1992\n337\nAmerican Society for Microbiology\nWashington D.C"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.58.4.1394-1397.1992"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.64.1.279-286.1998","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.64.1.279-286.1998","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T05:51:46Z","timestamp":1645595506000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.64.1.279-286.1998"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,1]]},"references-count":17,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1998,1]]}},"alternative-id":["10.1128\/AEM.64.1.279-286.1998"],"URL":"https:\/\/doi.org\/10.1128\/aem.64.1.279-286.1998","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,1]]},"assertion":[{"value":"1997-06-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1997-10-31","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"1998-01-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}