{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T06:04:38Z","timestamp":1791266678107,"version":"4.1.0"},"reference-count":27,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[1998,11,1]],"date-time":"1998-11-01T00:00:00Z","timestamp":909878400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[1998,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>For PCR detection of<jats:italic>Coxiella burnetii<\/jats:italic>in various clinical specimens we developed a sample preparation method in which silica binding of DNA was used. This method was found to be fast, easily performed with large numbers of samples, and equally sensitive for all of the specimens tested (livers, spleens, placentas, heart valves, milk, blood). The DNA preparation method described here can also be used as an initial step in any PCR-based examination of specimens. The procedure was tested with more than 600 milk samples, which were taken from 21 cows that were seropositive for<jats:italic>C. burnetii<\/jats:italic>and reportedly had fertility problems (and therefore were suspected of shedding the agent through milk intermittently or continuously). Of the 21 cows tested, 6 were shedding<jats:italic>C. burnetii<\/jats:italic>through milk. Altogether,<jats:italic>C. burnetii<\/jats:italic>DNA was detected in 6% of the samples. There was no correlation between the shedding pattern and the serological results.<\/jats:p>","DOI":"10.1128\/aem.64.11.4234-4237.1998","type":"journal-article","created":{"date-parts":[[2019,12,19]],"date-time":"2019-12-19T18:55:26Z","timestamp":1576781726000},"page":"4234-4237","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":65,"title":["PCR Detection of<i>Coxiella burnetii<\/i>from Different Clinical Specimens, Especially Bovine Milk, on the Basis of DNA Preparation with a Silica Matrix"],"prefix":"10.1128","volume":"64","author":[{"given":"Helga","family":"Lorenz","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Institute for Hygiene and Infectious Diseases of Animals, Justus-Liebig University, Giessen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cornelie","family":"Ja\u0308ger","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Institute for Hygiene and Infectious Diseases of Animals, Justus-Liebig University, Giessen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hermann","family":"Willems","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Institute for Hygiene and Infectious Diseases of Animals, Justus-Liebig University, Giessen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georg","family":"Baljer","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Institute for Hygiene and Infectious Diseases of Animals, Justus-Liebig University, Giessen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","first-page":"109","article-title":"Kontinuierliche Vermehrung von Coxiella burnetii durch persistierende Infektion in Buffalo-Green-Monkey (BGM)-Zellkulturen","volume":"30","author":"Arens M.","year":"1983","unstructured":"Arens M. 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