{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T06:09:16Z","timestamp":1779343756887,"version":"3.51.4"},"reference-count":23,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[1998,11,1]],"date-time":"1998-11-01T00:00:00Z","timestamp":909878400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[1998,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The rate, progress, and limits of trichloroethylene (TCE) degradation by\n            <jats:italic>Ralstonia eutropha<\/jats:italic>\n            AEK301\/pYK3021 whole cells were examined in the absence of aromatic induction. At TCE concentrations up to 800 \u03bcM, degradation rates were sustained until TCE was no longer detectable. The\n            <jats:italic>\n              K\n              <jats:sub>s<\/jats:sub>\n            <\/jats:italic>\n            and\n            <jats:italic>V<\/jats:italic>\n            <jats:sub>max<\/jats:sub>\n            for TCE degradation by AEK301\/pYK3021 whole cells were determined to be 630 \u03bcM and 22.6 nmol\/min\/mg of total protein, respectively. The sustained linear rates of TCE degradation by AEK301\/pYK3021 up to a concentration of 800 \u03bcM TCE suggest that solvent effects are limited during the degradation of TCE and that this construct is little affected by the formation of toxic intermediates at the TCE levels and assay duration tested. TCE degradation by this strain is subject to carbon catabolite repression.\n          <\/jats:p>","DOI":"10.1128\/aem.64.11.4353-4356.1998","type":"journal-article","created":{"date-parts":[[2019,12,19]],"date-time":"2019-12-19T18:55:30Z","timestamp":1576781730000},"page":"4353-4356","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":23,"title":["Whole-Cell Kinetics of Trichloroethylene Degradation by Phenol Hydroxylase in a\n            <i>Ralstonia eutropha<\/i>\n            JMP134 Derivative"],"prefix":"10.1128","volume":"64","author":[{"given":"Patricia J.","family":"Ayoubi","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Microbiology and Molecular Genetics, Oklahoma State University, Stillwater, Oklahoma 74078"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alan R.","family":"Harker","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Microbiology and Molecular Genetics, Oklahoma State University, Stillwater, Oklahoma 74078"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.64.1.1-6.1998"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(94)00844-I"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.mi.45.100191.001435"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.57.6.1602-1608.1991"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.56.5.1279-1285.1990"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1021\/es00156a012"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.56.4.1179-1181.1990"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.171.1.314-320.1989"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.60.12.4634-4637.1994"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.62.9.3227-3233.1996"},{"key":"e_1_3_1_12_2","unstructured":"Kim\nY.\nA recombinant approach to the isolation and characterization of a primary degrader of trichloroethylene. 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