{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T07:52:32Z","timestamp":1761897152891},"reference-count":26,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[1998,12,1]],"date-time":"1998-12-01T00:00:00Z","timestamp":912470400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[1998,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Bioluminescence, mRNA levels, and toluene degradation rates in\n            <jats:italic>Pseudomonas putida<\/jats:italic>\n            TVA8 were measured as a function of various concentrations of toluene and trichloroethylene (TCE). TVA8 showed an increasing bioluminescence response to increasing TCE and toluene concentrations. Compared to uninduced TVA8 cultures,\n            <jats:italic>todC1<\/jats:italic>\n            mRNA levels increased 11-fold for TCE-treated cultures and 13-fold for toluene-treated cultures. Compared to uninduced\n            <jats:italic>P. putida<\/jats:italic>\n            F1 cultures,\n            <jats:italic>todC1<\/jats:italic>\n            mRNA levels increased 4.4-fold for TCE-induced cultures and 4.9-fold for toluene-induced cultures. Initial toluene degradation rates were linearly correlated with specific bioluminescence in TVA8 cultures.\n          <\/jats:p>","DOI":"10.1128\/aem.64.12.5049-5052.1998","type":"journal-article","created":{"date-parts":[[2019,12,19]],"date-time":"2019-12-19T18:57:32Z","timestamp":1576781852000},"page":"5049-5052","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":42,"title":["Induction of the\n            <i>tod<\/i>\n            Operon by Trichloroethylene in\n            <i>Pseudomonas putida<\/i>\n            TVA8"],"prefix":"10.1128","volume":"64","author":[{"given":"Justin T.","family":"Shingleton","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Center for Environmental Biotechnology1 and"},{"name":"<!--label omitted: 2-->Department of Chemical Engineering,2 The University of Tennessee, Knoxville, Tennessee 37996"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruce M.","family":"Applegate","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Center for Environmental Biotechnology1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aaron C.","family":"Nagel","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Center for Environmental Biotechnology1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul R.","family":"Bienkowski","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Center for Environmental Biotechnology1 and"},{"name":"<!--label omitted: 2-->Department of Chemical Engineering,2 The University of Tennessee, Knoxville, Tennessee 37996"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gary S.","family":"Sayler","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Center for Environmental Biotechnology1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.64.7.2730-2735.1998"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jim.2900334"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/bit.260450509"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.7.1991"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.mi.45.100191.001435"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.56.5.1279-1285.1990"},{"key":"e_1_3_2_8_2","unstructured":"Gibson\nD. T.\nYeh\nW.\nLiu\nT.\nSubramanian\nV.\nToluene dioxygenase: a multicomponent enzyme system from Pseudomonas putida Oxygenases and oxygen metabolisms.\nNozaki\nM.\n1982\n51\n61\nAcademic Press Inc.\nNew York N.Y"},{"key":"e_1_3_2_9_2","unstructured":"Green\nD. W.\nPerry\u2019s chemical engineers\u2019 handbook\n6th ed.\n1984\n12\n45\nMcGraw-Hill Inc.\nNew York N.Y"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.60.12.4634-4637.1994"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7012(98)00043-8"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.58.6.1839-1846.1992"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.20.6955"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.62.3.825-833.1996"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.61.9.3479-3481.1995"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.55.11.2819-2826.1989"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1551-8833.1981.tb04680.x"},{"key":"e_1_3_2_18_2","first-page":"7.53","volume":"1","author":"Sambrook J.","year":"1989","unstructured":"Sambrook J. Fritsch E. F. Maniatis T. Molecular cloning: a laboratory manual 2nd ed. 1 1989 7.53 7.55 Cold Spring Harbor Laboratory Press Plainview, N.Y","journal-title":"Molecular cloning: a laboratory manual"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.37.5.878-885.1979"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-43-2-159"},{"key":"e_1_3_2_21_2","unstructured":"Stecher\nP. G.\nThe Merck index\n8th ed.\n1968\n1069\nMerck and Co. Inc.\nRahway N.J"},{"key":"e_1_3_2_22_2","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1021\/cen-v065n039.p011","article-title":"Chlorinated solvents use hurt by federal rules.","volume":"65","author":"Storck W.","year":"1987","unstructured":"Storck W. Chlorinated solvents use hurt by federal rules. Chem. Eng. News 65 1987 11","journal-title":"Chem. Eng. News"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.55.12.3155-3161.1989"},{"key":"e_1_3_2_24_2","unstructured":"Wackett\nL. P.\nBacterial co-metabolism of halogenated compounds\nMicrobial transformation and degradation of toxic chemicals.\nYoung\nL. Y.\nCerniglia\nC. E.\n1995\n217\n241\nWiley-Liss Inc.\nNew York N.Y"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.54.7.1703-1708.1988"},{"key":"e_1_3_2_26_2","first-page":"282","article-title":"Efficient degradation of trichloroethylene by a recombinant Escherichia coli.","volume":"7","author":"Winter R. B.","year":"1989","unstructured":"Winter R. B. Yen K.-M. Ensley B. D. Efficient degradation of trichloroethylene by a recombinant Escherichia coli. Biotechnology 7 1989 282 285","journal-title":"Biotechnology"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.55.12.3162-3166.1989"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.64.12.5049-5052.1998","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.64.12.5049-5052.1998","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T05:35:54Z","timestamp":1645594554000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.64.12.5049-5052.1998"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,12]]},"references-count":26,"journal-issue":{"issue":"12","published-print":{"date-parts":[[1998,12]]}},"alternative-id":["10.1128\/AEM.64.12.5049-5052.1998"],"URL":"https:\/\/doi.org\/10.1128\/aem.64.12.5049-5052.1998","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,12]]},"assertion":[{"value":"1998-07-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1998-09-28","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"1998-12-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}