{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T06:20:54Z","timestamp":1790317254488,"version":"4.1.0"},"reference-count":37,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[1998,4,1]],"date-time":"1998-04-01T00:00:00Z","timestamp":891388800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[1998,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Two membrane-bound, reductive dehalogenases that constitute a novel pathway for complete dechlorination of tetrachloroethene (perchloroethylene [PCE]) to ethene were partially purified from an anaerobic microbial enrichment culture containing\n            <jats:italic>Dehalococcoides ethenogenes<\/jats:italic>\n            195. When titanium(III) citrate and methyl viologen were used as reductants, PCE-reductive dehalogenase (PCE-RDase) (51 kDa) dechlorinated PCE to trichloroethene (TCE) at a rate of 20 \u03bcmol\/min\/mg of protein. TCE-reductive dehalogenase (TCE-RDase) (61 kDa) dechlorinated TCE to ethene. TCE,\n            <jats:italic>cis<\/jats:italic>\n            -1,2-dichloroethene, and 1,1-dichloroethene were dechlorinated at similar rates, 8 to 12 \u03bcmol\/min\/mg of protein. Vinyl chloride and\n            <jats:italic>trans<\/jats:italic>\n            -1,2-dichloroethene were degraded at rates which were approximately 2 orders of magnitude lower. The light-reversible inhibition of TCE-RDase by iodopropane and the light-reversible inhibition of PCE-RDase by iodoethane suggest that both of these dehalogenases contain Co(I) corrinoid cofactors. Isolation and characterization of these novel bacterial enzymes provided further insight into the catalytic mechanisms of biological reductive dehalogenation.\n          <\/jats:p>","DOI":"10.1128\/aem.64.4.1270-1275.1998","type":"journal-article","created":{"date-parts":[[2019,12,19]],"date-time":"2019-12-19T19:33:17Z","timestamp":1576783997000},"page":"1270-1275","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":244,"title":["Reductive Dechlorination of Tetrachloroethene to Ethene by a Two-Component Enzyme Pathway"],"prefix":"10.1128","volume":"64","author":[{"given":"Jon K.","family":"Magnuson","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->USAF Armstrong Laboratory, Environics Directorate, Tyndall Air Force Base, Florida 32403-5323,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert V.","family":"Stern","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->USAF Armstrong Laboratory, Environics Directorate, Tyndall Air Force Base, Florida 32403-5323,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James M.","family":"Gossett","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->School of Civil and Environmental Engineering,2 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen H.","family":"Zinder","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->Section of Microbiology,3 Cornell University, Ithaca, New York 14853"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David R.","family":"Burris","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->USAF Armstrong Laboratory, Environics Directorate, Tyndall Air Force Base, Florida 32403-5323,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.56.8.2511-2516.1990"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1021\/es00158a005"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.45.4.1286-1294.1983"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)97287-X"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1021\/es960116o"},{"key":"e_1_3_2_8_2","unstructured":"Chrambach\nA.\nThe practice of quantitative gel electrophoresis.\n1985\nVCH Publishers\nDeerfield Beach Fla"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.58.6.1996-2000.1992"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.56.10.2999-3005.1990"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.57.8.2287-2292.1991"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.mi.45.100191.001435"},{"key":"e_1_3_2_13_2","first-page":"22062","article-title":"Environmental Protection Agency, National Primary and Secondary Drinking Water Regulations.","volume":"54","author":"Federal Register","year":"1989","unstructured":"Federal Register Environmental Protection Agency, National Primary and Secondary Drinking Water Regulations. 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