{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T06:57:29Z","timestamp":1789628249616,"version":"build-2803163510"},"reference-count":27,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[1998,5,1]],"date-time":"1998-05-01T00:00:00Z","timestamp":893980800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[1998,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            During the last 100 years, the neuston bacterium\n            <jats:italic>Nevskia ramosa<\/jats:italic>\n            has been described several times. This bacterium forms conspicuous rosette-like microcolonies at the air-water interface. In this study, pure cultures of\n            <jats:italic>Nevskia ramosa<\/jats:italic>\n            were obtained for the first time, from a bog lake (strain Soe1, DSMZ 11499\n            <jats:sup>T<\/jats:sup>\n            ) and a freshwater ditch (strain OL1, DSMZ 11500). The isolates showed special adaptations to life in the epineuston. They formed hydrophobic surface films with a dull appearance.\n            <jats:italic>N. ramosa<\/jats:italic>\n            is sensitive to UV radiation but revealed a very effective photorepair mechanism. Exposure to light at a wavelength of 350 nm after UV treatment raised the number of surviving cells by several orders of magnitude. The isolates grew with a broad range of organic substrates. Surface films were formed only in the absence of combined nitrogen; however, nitrogenase activity was not detected. It appears that during growth at the air-water interface the cells benefit from trapping ammonia from the air. The G+C content of the DNA was 67.8 and 69.0 mol% for strains Soe1 and OL1, respectively. The slight difference was confirmed by enterobacterial repetitive intergenic consensus PCR. The 16S rRNA sequences revealed 99.2% similarity. Thus, both isolates belong to the same species. The phylogenetic analysis indicated that\n            <jats:italic>Nevskia<\/jats:italic>\n            is a member of the gamma-subclass\n            <jats:italic>Proteobacteria<\/jats:italic>\n            that has no known close relatives.\n          <\/jats:p>","DOI":"10.1128\/aem.64.5.1890-1894.1998","type":"journal-article","created":{"date-parts":[[2019,12,19]],"date-time":"2019-12-19T19:35:29Z","timestamp":1576784129000},"page":"1890-1894","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":49,"title":["Ecophysiological and Phylogenetic Studies of\n            <i>Nevskia ramosa<\/i>\n            in Pure Culture"],"prefix":"10.1128","volume":"64","author":[{"given":"Heike","family":"St\u00fcrmeyer","sequence":"first","affiliation":[{"name":"Institut f\u00fcr Chemie und Biologie des Meeres, Oldenburg,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f6rg","family":"Overmann","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Chemie und Biologie des Meeres, Oldenburg,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans-Dietrich","family":"Babenzien","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Gew\u00e4sser\u00f6kologie und Binnenfischerei, Neuglobsow,2 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heribert","family":"Cypionka","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Chemie und Biologie des Meeres, Oldenburg,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"111","article-title":"\u00dcber Vorkommen und Kultur von Nevskia ramosa","volume":"1","author":"Babenzien H.-D.","year":"1965","unstructured":"Babenzien H.-D. (1965) \u00dcber Vorkommen und Kultur von Nevskia ramosa.Zentbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 1 Suppl.1:111\u2013116.","journal-title":"Zentbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 1 Suppl."},{"key":"e_1_3_2_3_2","first-page":"1503","article-title":"Untersuchungen zur Mikrobiologie des Neustons","volume":"16","author":"Babenzien H.-D.","year":"1966","unstructured":"Babenzien H.-D. (1966) Untersuchungen zur Mikrobiologie des Neustons.Verh. Int. Ver. Limnol.16:1503\u20131511.","journal-title":"Verh. Int. Ver. Limnol."},{"key":"e_1_3_2_4_2","first-page":"89","article-title":"Zur Biologie von Nevskia ramosa","volume":"7","author":"Babenzien H.-D.","year":"1967","unstructured":"Babenzien H.-D. (1967) Zur Biologie von Nevskia ramosa.Z. Allg. Mikrobiol.7:89\u201396.","journal-title":"Z. Allg. Mikrobiol."},{"key":"e_1_3_2_5_2","first-page":"1979","article-title":"Genus Nevskia Famintzin 1892, 484AL","volume":"3","author":"Babenzien H.-D.","year":"1989","unstructured":"Babenzien H.-D. (1989) Genus Nevskia Famintzin 1892, 484AL. in Bergey\u2019s manual of systematic bacteriology, eds StaleyJ. T.BryantM. P.PfennigN.HoltJ. G. (Williams & Wilkins, Baltimore, Md), 3:1979\u20131981.","journal-title":"Bergey\u2019s manual of systematic bacteriology"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0723-2020(11)80471-6"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/21.5.1199"},{"key":"e_1_3_2_8_2","first-page":"481","article-title":"Eine neue Bakterienform: Nevskia ramosa","volume":"34","author":"Famintzin A.","year":"1892","unstructured":"Famintzin A. (1892) Eine neue Bakterienform: Nevskia ramosa.Bull. Acad. Imp. Sci. St. Petersburg, New Ser. 234:481\u2013468.","journal-title":"Bull. Acad. Imp. Sci. St. Petersburg, New Ser. 2"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-42-1-166"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.64.5.1895-1901.1998"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-139-7-1531"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1139\/m86-019"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.30.1.61-93.1935"},{"key":"e_1_3_2_14_2","unstructured":"Hippe H. Personal communication."},{"key":"e_1_3_2_15_2","doi-asserted-by":"crossref","unstructured":"Hirsch\nP.\n (1981) The genus Nevskia . in The prokaryotes. A handbook on habitats isolation and identification of bacteria. eds StarrM. P.StolpH.Tr\u00fcperH. G.BalowsA.SchlegelH. G. (Springer-Verlag Berlin Germany) pp 521\u2013523.","DOI":"10.1007\/978-3-662-13187-9_42"},{"key":"e_1_3_2_16_2","doi-asserted-by":"crossref","unstructured":"Jukes\nT. H.\nCantor\nC. 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(Georg Thieme Verlag Stuttgart Germany)."},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00414460"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/19.24.6823"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.64.5.1890-1894.1998","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.64.5.1890-1894.1998","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T01:35:53Z","timestamp":1645580153000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.64.5.1890-1894.1998"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,5]]},"references-count":27,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1998,5]]}},"alternative-id":["10.1128\/AEM.64.5.1890-1894.1998"],"URL":"https:\/\/doi.org\/10.1128\/aem.64.5.1890-1894.1998","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,5]]},"assertion":[{"value":"1997-09-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1998-02-17","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"1998-05-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}