{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,4]],"date-time":"2025-06-04T21:13:51Z","timestamp":1749071631011},"reference-count":55,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[1998,7,1]],"date-time":"1998-07-01T00:00:00Z","timestamp":899251200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[1998,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Pseudomonas<\/jats:italic>\n            sp. strain JR1 exhibits trichloroethene (TCE) oxidation activity with isopropylbenzene (IPB) as the inducer substrate. We previously reported the genes encoding the first three enzymes of the IPB-degradative pathway (\n            <jats:italic>ipbA1<\/jats:italic>\n            ,\n            <jats:italic>ipbA2<\/jats:italic>\n            ,\n            <jats:italic>ipbA3<\/jats:italic>\n            ,\n            <jats:italic>ipbA4<\/jats:italic>\n            ,\n            <jats:italic>ipbB<\/jats:italic>\n            , and\n            <jats:italic>ipbC<\/jats:italic>\n            ) and identified the initial IPB dioxygenase (IpbA1A2A3A4) as responsible for TCE cooxidation (U. Pflugmacher, B. Averhoff, and G. Gottschalk, Appl. Environ. Microbiol. 62:3967\u20133977, 1996). Primer extension analyses revealed multiple transcriptional start points located upstream of the translational initiation codon of\n            <jats:italic>ipbA1<\/jats:italic>\n            . The transcription from these start sites was found to be IPB dependent. Thirty-one base pairs upstream of the first transcriptional start point tandemly repeated DNA sequences overlapping the \u221235 region of a putative \u03c2\n            <jats:sup>70<\/jats:sup>\n            promoter were found. These repeats exhibit significant sequence similarity to the operator-promoter region of the\n            <jats:italic>xyl meta<\/jats:italic>\n            operon in\n            <jats:italic>Pseudomonas putida<\/jats:italic>\n            , which is required for the binding of XylS, a regulatory protein of the XylS (also called AraC) family. These similarities suggest that the transcription of the IPB dioxygenase genes is modulated by a regulatory protein of the XylS\/AraC family. The construction of an\n            <jats:italic>ipb<\/jats:italic>\n            DNA module devoid of this\n            <jats:italic>ipb<\/jats:italic>\n            operator-promoter region and the stable insertion of this DNA module into the genomes of different\n            <jats:italic>Pseudomonas<\/jats:italic>\n            strains resulted in pseudomonads with constitutive IPB and TCE oxidation activities. Constitutive TCE oxidation of two such\n            <jats:italic>Pseudomonas<\/jats:italic>\n            hybrid strains, JR1A::\n            <jats:italic>ipb<\/jats:italic>\n            and CBS-3::\n            <jats:italic>ipb<\/jats:italic>\n            , was found to be stable for more than 120 generations in antibiotic-free medium. Evaluation of constitutive TCE degradation rates revealed that continuous cultivation of strain JR1A::\n            <jats:italic>ipb<\/jats:italic>\n            resulted in a significant increase in rates of TCE degradation.\n          <\/jats:p>","DOI":"10.1128\/aem.64.7.2454-2462.1998","type":"journal-article","created":{"date-parts":[[2019,12,19]],"date-time":"2019-12-19T19:37:28Z","timestamp":1576784248000},"page":"2454-2462","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":9,"title":["Construction and Use of an\n            <i>ipb<\/i>\n            DNA Module To Generate\n            <i>Pseudomonas<\/i>\n            Strains with Constitutive Trichloroethene and Isopropylbenzene Oxidation Activity"],"prefix":"10.1128","volume":"64","author":[{"given":"Frank","family":"Berendes","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Institut fu\u0308r Mikrobiologie und Genetik, Georg-August-Universita\u0308t, 37077 Go\u0308ttingen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicolas","family":"Sabarth","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Institut fu\u0308r Mikrobiologie und Genetik, Georg-August-Universita\u0308t, 37077 Go\u0308ttingen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beate","family":"Averhoff","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Institut fu\u0308r Mikrobiologie und Genetik, Georg-August-Universita\u0308t, 37077 Go\u0308ttingen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gerhard","family":"Gottschalk","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Institut fu\u0308r Mikrobiologie und Genetik, Georg-August-Universita\u0308t, 37077 Go\u0308ttingen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.57.1.228-235.1991"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(89)90042-9"},{"key":"e_1_3_2_4_2","unstructured":"Bachmann\nB. J.\nLinkage map of Escherichia coli K-12\nEscherichia coli and Salmonella typhimurium: cellular and molecular biology.\nNeidhardt\nC.\nIngraham\nJ. L.\nLow\nK. B.\nMagasanik\nB.\nSchaechter\nM.\nUmbarger\nH. E.\n1987\n807\n876\nAmerican Society for Microbiology\nWashington D.C"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj1010293"},{"key":"e_1_3_2_6_2","first-page":"376","article-title":"XL1-blue: a high efficiency plasmid transforming recA Escherichia coli strain with beta-galactosidase selection.","volume":"5","author":"Bullock W. O.","year":"1987","unstructured":"Bullock W. O. Fernandez J. M. Short J. M. XL1-blue: a high efficiency plasmid transforming recA Escherichia coli strain with beta-galactosidase selection. BioTechniques 5 1987 376 378","journal-title":"BioTechniques"},{"key":"e_1_3_2_7_2","unstructured":"Claus\nD.\nLack\nP.\nNeu\nB.\nDeutsche Sammlung von Mikroorganismen. Catalogue of strains\n3rd ed.\n1983\nGesellschaft f\u00fcr Biotechnologische Forschung mbH\nBraunschweig Germany"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00701455"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00249058"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.172.11.6568-6572.1990"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/12.1Part1.387"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.176.8.2354-2361.1994"},{"key":"e_1_3_2_13_2","unstructured":"Eaton R. W. Personal communication."},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.58.9.3038-3046.1992"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00276540"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.57.6.1602-1608.1991"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.56.5.1279-1285.1990"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.176.7.2121-2123.1994"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(83)80284-8"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.56.4.1179-1181.1990"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.57.1.236-244.1991"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/ange.19941061905"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.172.11.6557-6567.1990"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.176.6.1773-1776.1994"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(86)90187-3"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.60.7.2473-2482.1994"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.112.2.917-931.1972"},{"key":"e_1_3_2_28_2","unstructured":"Kaphammer\nB.\nKukor\nJ. J.\nOlsen\nR. H.\nCloning and characterization of a novel toluene degradative pathway from Pseudomonas pickettii PKO1 abstr. K-145\nAbstracts of the 90th Annual Meeting of the American Society for Microbiology 1990.\n1990\n243\nAmerican Society for Microbiology\nWashington D.C"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1099\/13500872-142-11-3241"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00282749"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1993.1189"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.59.4.960-967.1993"},{"key":"e_1_3_2_33_2","first-page":"443","article-title":"Trichloroethylene oxidation by toluene dioxygenase.","volume":"158","author":"Li S.","year":"1992","unstructured":"Li S. Wackett L. P. Trichloroethylene oxidation by toluene dioxygenase. Biochem. Biophys. Res. Commun. 158 1992 443 451","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(70)90051-3"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4781(93)90149-8"},{"key":"e_1_3_2_36_2","unstructured":"Michel\nJ.\nUntersuchungen zur Regulation des Isopropylbenzolabbauweges in Pseudomonas sp. JR1. Diploma thesis.\n1995\nUniversity of G\u00f6ttingen\nG\u00f6ttingen Germany"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00534a041"},{"key":"e_1_3_2_38_2","first-page":"1145","article-title":"Metabolism of trichloroethylene in isolated hepatocytes, microsomes, and reconstituted enzyme systems containing cytochrome P-450.","volume":"43","author":"Miller R. E.","year":"1983","unstructured":"Miller R. E. Guengerich F. P. Metabolism of trichloroethylene in isolated hepatocytes, microsomes, and reconstituted enzyme systems containing cytochrome P-450. Cancer Res. 43 1983 1145 1152","journal-title":"Cancer Res."},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.170.6.2575-2583.1988"},{"key":"e_1_3_2_40_2","unstructured":"National Cancer Institute\nCarcinogenesis bioassay of trichloroethylene. Chemical Abstracts no. 79-01-6. U.S. Department of Health Education and Welfare publication (NIH) 76-802. U.S.\n1976\nDepartment of Health Education and Welfare\nWashington D.C"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-7012(90)90050-G"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.57.1.7-14.1991"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.62.11.3967-3977.1996"},{"key":"e_1_3_2_44_2","unstructured":"Sambrook\nJ.\nFritsch\nE. F.\nManiatis\nT.\nMolecular cloning: a laboratory manual\n2nd ed.\n1989\nCold Spring Harbor Laboratory\nCold Spring Harbor N.Y"},{"key":"e_1_3_2_45_2","first-page":"8934","article-title":"DNA sequencing with chain-terminating inhibitors.","volume":"85","author":"Sanger F.","year":"1977","unstructured":"Sanger F. Nicklen S. Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 85 1977 8934 8938","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"e_1_3_2_46_2","first-page":"117","article-title":"Die Carotinoide der Thiorhodaceae.","volume":"46","author":"Schmidt K.","year":"1963","unstructured":"Schmidt K. Liaanen-Jensen S. Schlegel H. G. Die Carotinoide der Thiorhodaceae. Arch. Microbiol. 46 1963 117 126","journal-title":"Arch. Microbiol."},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.58.12.3977-3983.1992"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.57.7.1935-1941.1991"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.178.14.4039-4046.1996"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1987.tb02180.x"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00414460"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.54.7.1703-1708.1988"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.55.10.2723-2725.1989"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.55.11.2960-2964.1989"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1038\/368627a0"},{"key":"e_1_3_2_56_2","first-page":"282","article-title":"Efficient degradation of trichloroethylene by a recombinant Escherichia coli.","volume":"7","author":"Winter R. B.","year":"1989","unstructured":"Winter R. B. Yen K.-M. Ensley B. D. Efficient degradation of trichloroethylene by a recombinant Escherichia coli. Bio\/Technology 7 1989 282 285","journal-title":"Bio\/Technology"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.64.7.2454-2462.1998","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.64.7.2454-2462.1998","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T06:19:36Z","timestamp":1645597176000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.64.7.2454-2462.1998"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,7]]},"references-count":55,"journal-issue":{"issue":"7","published-print":{"date-parts":[[1998,7]]}},"alternative-id":["10.1128\/AEM.64.7.2454-2462.1998"],"URL":"https:\/\/doi.org\/10.1128\/aem.64.7.2454-2462.1998","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,7]]},"assertion":[{"value":"1998-01-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1998-04-27","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"1998-07-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}