{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T06:35:35Z","timestamp":1791182135750,"version":"4.1.0"},"reference-count":48,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[1998,8,1]],"date-time":"1998-08-01T00:00:00Z","timestamp":901929600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[1998,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We characterized the regulated activity of the lactococcal\n            <jats:italic>nisA<\/jats:italic>\n            promoter in strains of the gram-positive species\n            <jats:italic>Streptococcus pyogenes<\/jats:italic>\n            ,\n            <jats:italic>Streptococcus agalactiae<\/jats:italic>\n            ,\n            <jats:italic>Streptococcus pneumoniae<\/jats:italic>\n            ,\n            <jats:italic>Enterococcus faecalis<\/jats:italic>\n            , and\n            <jats:italic>Bacillus subtilis. nisA<\/jats:italic>\n            promoter activity was dependent on the proteins NisR and NisK, which constitute a two-component signal transduction system that responds to the extracellular inducer nisin. The nisin sensitivity and inducer concentration required for maximal induction varied among the strains. Significant induction of the\n            <jats:italic>nisA<\/jats:italic>\n            promoter (10- to 60-fold induction) was obtained in all of the species studied at a nisin concentration just below the concentration at which growth is inhibited. The efficiency of the\n            <jats:italic>nisA<\/jats:italic>\n            promoter was compared to the efficiencies of the\n            <jats:italic>Spac<\/jats:italic>\n            ,\n            <jats:italic>xylA<\/jats:italic>\n            , and\n            <jats:italic>lacA<\/jats:italic>\n            promoters in\n            <jats:italic>B. subtilis<\/jats:italic>\n            and in\n            <jats:italic>S. pyogenes<\/jats:italic>\n            . Because\n            <jats:italic>nisA<\/jats:italic>\n            promoter-driven expression is regulated in many gram-positive bacteria, we expect it to be useful for genetic studies, especially studies with pathogenic streptococci in which no other regulated promoters have been described.\n          <\/jats:p>","DOI":"10.1128\/aem.64.8.2763-2769.1998","type":"journal-article","created":{"date-parts":[[2019,12,19]],"date-time":"2019-12-19T19:38:31Z","timestamp":1576784311000},"page":"2763-2769","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":149,"title":["Use of the Lactococcal\n            <i>nisA<\/i>\n            Promoter To Regulate Gene Expression in Gram-Positive Bacteria: Comparison of Induction Level and Promoter Strength"],"prefix":"10.1128","volume":"64","author":[{"given":"Zehava","family":"Eichenbaum","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Microbiology and Immunology, Emory University Health Sciences Center, Atlanta, Georgia 30322,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J.","family":"Federle","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Microbiology and Immunology, Emory University Health Sciences Center, Atlanta, Georgia 30322,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana","family":"Marra","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Microbiology and Immunology, Emory University Health Sciences Center, Atlanta, Georgia 30322,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Willem M.","family":"de Vos","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Department of Biophysical Chemistry, Section of Microbial Ingredients, Netherlands Institute for Dairy Research (NIZO), 6710 BA Ede, The Netherlands2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oscar P.","family":"Kuipers","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Department of Biophysical Chemistry, Section of Microbial Ingredients, Netherlands Institute for Dairy Research (NIZO), 6710 BA Ede, The Netherlands2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michiel","family":"Kleerebezem","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Department of Biophysical Chemistry, Section of Microbial Ingredients, Netherlands Institute for Dairy Research (NIZO), 6710 BA Ede, The Netherlands2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"June R.","family":"Scott","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Microbiology and Immunology, Emory University Health Sciences Center, Atlanta, Georgia 30322,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","unstructured":"Arigoni F. 1998. 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