{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T02:12:49Z","timestamp":1778551969896,"version":"3.51.4"},"reference-count":17,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[1998,8,1]],"date-time":"1998-08-01T00:00:00Z","timestamp":901929600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[1998,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Toluene\/\n            <jats:italic>o<\/jats:italic>\n            -xylene monooxygenase (ToMO) from\n            <jats:italic>Pseudomonas stutzeri<\/jats:italic>\n            OX1, which oxidizes toluene and\n            <jats:italic>o<\/jats:italic>\n            -xylene, was examined for its ability to degrade the environmental pollutants trichloroethylene (TCE), 1,1-dichloroethylene (1,1-DCE),\n            <jats:italic>cis<\/jats:italic>\n            -1,2-DCE,\n            <jats:italic>trans<\/jats:italic>\n            -1,2-DCE, chloroform, dichloromethane, phenol, 2,4-dichlorophenol, 2,4,5-trichlorophenol, 2,4,6-trichlorophenol, 2,3,5,6-tetrachlorophenol, and 2,3,4,5,6-pentachlorophenol.\n            <jats:italic>Escherichia coli<\/jats:italic>\n            JM109 that expressed ToMO from genes on plasmid pBZ1260 under control of the\n            <jats:italic>lac<\/jats:italic>\n            promoter degraded TCE (3.3 \u03bcM), 1,1-DCE (1.25 \u03bcM), and chloroform (6.3 \u03bcM) at initial rates of 3.1, 3.6, and 1.6 nmol\/(min \u00b7 mg of protein), respectively. Stoichiometric amounts of chloride release were seen, indicating mineralization (2.6, 1.5, and 2.3 Cl\n            <jats:sup>\u2212<\/jats:sup>\n            atoms per molecule of TCE, 1,1-DCE, and chloroform, respectively). Thus, the substrate range of ToMO is extended to include aliphatic chlorinated compounds.\n          <\/jats:p>","DOI":"10.1128\/aem.64.8.3023-3024.1998","type":"journal-article","created":{"date-parts":[[2019,12,19]],"date-time":"2019-12-19T19:39:28Z","timestamp":1576784368000},"page":"3023-3024","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":60,"title":["Oxidation of Trichloroethylene, 1,1-Dichloroethylene, and Chloroform by Toluene\/\n            <i>o<\/i>\n            -Xylene Monooxygenase from\n            <i>Pseudomonas stutzeri<\/i>\n            OX1"],"prefix":"10.1128","volume":"64","author":[{"given":"Sadhana","family":"Chauhan","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Chemical and Biochemical Engineering, University of California, Irvine, California 92697-2575,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paola","family":"Barbieri","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Dipartimento di Genetica e di Biologia dei Microrganismi, Universita\u0300 degli studi di Milano, 20133 Milan, Italy2"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas K.","family":"Wood","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Chemical and Biochemical Engineering, University of California, Irvine, California 92697-2575,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.53.9.2129-2132.1987"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac60122a018"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.62.10.3704-3711.1996"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-143-7-2321"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(94)00844-I"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.56.5.1279-1285.1990"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.60.7.2473-2482.1994"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.12.3858-3865.1997"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.62.3.825-833.1996"},{"key":"e_1_3_2_11_2","unstructured":"Maniatis\nT.\nFritsch\nE. 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