{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T07:49:35Z","timestamp":1791186575263,"version":"4.1.0"},"reference-count":34,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[1998,9,1]],"date-time":"1998-09-01T00:00:00Z","timestamp":904608000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[1998,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The effect of nitrogen source on methane-oxidizing bacteria with respect to cellular growth and trichloroethylene (TCE) degradation ability were examined. One mixed chemostat culture and two pure type II methane-oxidizing strains,\n            <jats:italic>Methylosinus trichosporium<\/jats:italic>\n            OB3b and strain CAC-2, which was isolated from the chemostat culture, were used in this study. All cultures were able to grow with each of three different nitrogen sources: ammonia, nitrate, and molecular nitrogen. Both\n            <jats:italic>M. trichosporium<\/jats:italic>\n            OB3b and strain CAC-2 showed slightly lower net cellular growth rates and cell yields but exhibited higher methane uptake rates, levels of poly-\u03b2-hydroxybutyrate (PHB) production, and naphthalene oxidation rates when grown under nitrogen-fixing conditions. The TCE-degrading ability of each culture was measured in terms of initial TCE oxidation rates and TCE transformation capacities (mass of TCE degraded\/biomass inactivated), measured both with and without external energy sources. Higher initial TCE oxidation rates and TCE transformation capacities were observed in nitrogen-fixing mixed,\n            <jats:italic>M. trichosporium<\/jats:italic>\n            OB3b, and CAC-2 cultures than in nitrate- or ammonia-supplied cells. TCE transformation capacities were found to correlate with cellular PHB content in all three cultures. The results of this study suggest that the nitrogen-fixing capabilities of methane-oxidizing bacteria can be used to select for high-activity TCE degraders for the enhancement of bioremediation in fixed-nitrogen-limited environments.\n          <\/jats:p>","DOI":"10.1128\/aem.64.9.3451-3457.1998","type":"journal-article","created":{"date-parts":[[2019,12,19]],"date-time":"2019-12-19T19:41:13Z","timestamp":1576784473000},"page":"3451-3457","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":45,"title":["Effect of Nitrogen Source on Growth and Trichloroethylene Degradation by Methane-Oxidizing Bacteria"],"prefix":"10.1128","volume":"64","author":[{"given":"Kung-Hui","family":"Chu","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Civil and Environmental Engineering, University of California at Berkeley, Berkeley, California 94720-1710"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa","family":"Alvarez-Cohen","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Civil and Environmental Engineering, University of California at Berkeley, Berkeley, California 94720-1710"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.57.1.228-235.1991"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0385-6380(86)90118-4"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3894(94)00119-2"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.56.8.2488-2493.1990"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00117048"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.62.9.3371-3377.1996"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.2175\/106143096X127235"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00425127"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.88.2.468-472.1964"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2911(08)60088-0"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00394121"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-83-1-113"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.51.4.720-724.1986"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1021\/es00156a012"},{"key":"e_1_3_2_16_2","unstructured":"Hazen\nT. 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