{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T12:01:25Z","timestamp":1759147285679},"reference-count":42,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[1999,11,1]],"date-time":"1999-11-01T00:00:00Z","timestamp":941414400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[1999,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Verotoxigenic\n            <jats:italic>Escherichia coli<\/jats:italic>\n            (VTEC) strains were isolated from food and animal fecal samples by using PCR to screen for the presence of VTEC after broth enrichment and then filtering VTEC-positive cultures through hydrophobic-grid membrane filters (HGMFs) which were incubated on MacConkey agar. The filters were probed with a digoxigenin-labeled PCR product generated by amplification of a conserved verotoxin gene sequence. Replication of the growth on filters allowed probe-positive colonies to be picked. When ground beef samples were inoculated with VTEC strains, 100% of the strains were recovered, and the detection limit was 0.1 CFU per g. Similar results were obtained with seven types of artificially contaminated vegetables. A survey of 32 packages of vegetables and 23 samples of apple cider obtained at the retail level did not reveal the presence of VTEC. However, the intestinal fecal contents of a moose, 1 of 35 wild mammals and birds examined, contained\n            <jats:italic>E. coli<\/jats:italic>\n            O157:H7. The DNA hybridization-HGMF method was also used in a prevalence survey of 327 raw and 744 ready-to-eat products; VTEC strains were recovered from 4.9% of the raw products and 0.7% of the ready-to-eat products. No serotype O157:H7 strains were detected. This method is particularly suited for surveys in which low numbers of VTEC-positive samples are expected and isolates are required.\n          <\/jats:p>","DOI":"10.1128\/aem.65.11.4775-4780.1999","type":"journal-article","created":{"date-parts":[[2019,12,19]],"date-time":"2019-12-19T19:43:36Z","timestamp":1576784616000},"page":"4775-4780","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":26,"title":["Application of a DNA Hybridization\u2013Hydrophobic-Grid Membrane Filter Method for Detection and Isolation of Verotoxigenic\n            <i>Escherichia coli<\/i>"],"prefix":"10.1128","volume":"65","author":[{"given":"E. C. D.","family":"Todd","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Bureau of Microbial Hazards, Food Directorate, Health Protection Branch, Health Canada, Ottawa, Ontario K1A 0L2,1"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. A.","family":"Szabo","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Bureau of Microbial Hazards, Food Directorate, Health Protection Branch, Health Canada, Ottawa, Ontario K1A 0L2,1"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. M.","family":"MacKenzie","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Bureau of Microbial Hazards, Food Directorate, Health Protection Branch, Health Canada, Ottawa, Ontario K1A 0L2,1"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Martin","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Education, Research and Laboratories Division, Ontario Ministry of Agriculture, Food and Rural Affairs, Guelph, Ontario N1G 4Y2,2"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Rahn","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->Guelph Laboratory, Health Protection Branch, Health Canada, Guelph, Ontario, N1G 3W4,3"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Gyles","sequence":"additional","affiliation":[{"name":"<!--label omitted: 4-->Department of Pathobiology, University of Guelph, Guelph, Ontario N1G 2W1,4"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Gao","sequence":"additional","affiliation":[{"name":"<!--label omitted: 4-->Department of Pathobiology, University of Guelph, Guelph, Ontario N1G 2W1,4"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Alves","sequence":"additional","affiliation":[{"name":"<!--label omitted: 5-->Livestock Technology Health Management, Ontario Ministry of Agriculture, Food and Rural Affairs, Fergus, Ontario N1M 2W3,5and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. J.","family":"Yee","sequence":"additional","affiliation":[{"name":"<!--label omitted: 6-->Laboratory Services Division, University of Guelph, Guelph, Ontario N1H 8J7,6 Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01709361"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/165.5.979"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-0813.1996.tb09948.x"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0404.980415"},{"key":"e_1_3_2_6_2","first-page":"70","article-title":"Enterohaemorrhagic Escherichia coli outbreak in South Australia associated with the consumption of mettwurst.","volume":"19","author":"Cameron S.","year":"1995","unstructured":"Cameron S. Walker C. Beers M. Rose N. Akear E. Enterohaemorrhagic Escherichia coli outbreak in South Australia associated with the consumption of mettwurst. 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