{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T22:23:51Z","timestamp":1762899831467},"reference-count":40,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[1999,3,1]],"date-time":"1999-03-01T00:00:00Z","timestamp":920246400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[1999,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The influence of modified plasma membrane fatty acid composition on cellular strontium accumulation in\n            <jats:italic>Saccharomyces cerevisiae<\/jats:italic>\n            was investigated. Growth of\n            <jats:italic>S. cerevisiae<\/jats:italic>\n            in the presence of 1 mM linoleate (18:2) (which results in 18:2 incorporation to \u223c70% of total cellular and plasma membrane fatty acids, with no effect on growth rate) yielded cells that accumulated Sr\n            <jats:sup>2+<\/jats:sup>\n            intracellularly at approximately twice the rate of\n            <jats:italic>S. cerevisiae<\/jats:italic>\n            grown without a fatty acid supplement. This effect was evident over a wide range of external Sr\n            <jats:sup>2+<\/jats:sup>\n            concentrations (25 \u03bcM to 5 mM) and increased with the extent of cellular 18:2 incorporation. Stimulation of Sr\n            <jats:sup>2+<\/jats:sup>\n            accumulation was not evident following enrichment of\n            <jats:italic>S. cerevisiae<\/jats:italic>\n            with either palmitoleate (16:1), linolenate (18:3) (\n            <jats:italic>n<\/jats:italic>\n            -3 and\n            <jats:italic>n<\/jats:italic>\n            -6 isomers), or eicosadienoate (20:2) (\n            <jats:italic>n<\/jats:italic>\n            -6 and\n            <jats:italic>n<\/jats:italic>\n            -9 isomers). Competition experiments revealed that Ca\n            <jats:sup>2+<\/jats:sup>\n            - and Mg\n            <jats:sup>2+<\/jats:sup>\n            -induced inhibition of Sr\n            <jats:sup>2+<\/jats:sup>\n            accumulation did not differ between unsupplemented and 18:2-supplemented cells. Treatment with trifluoperazine (TFP) (which can act as a calmodulin antagonist and Ca\n            <jats:sup>2+<\/jats:sup>\n            -ATPase inhibitor), at a low concentration that precluded nonspecific K\n            <jats:sup>+<\/jats:sup>\n            efflux, increased intracellular Sr\n            <jats:sup>2+<\/jats:sup>\n            accumulation by approximately 3.6- and 1.4-fold in unsupplemented and 18:2-supplemented cells, respectively. Thus, TFP abolished the enhanced Sr\n            <jats:sup>2+<\/jats:sup>\n            accumulation ability of 18:2-supplemented cells. Moreover, the rate of Sr\n            <jats:sup>2+<\/jats:sup>\n            release from Sr\n            <jats:sup>2+<\/jats:sup>\n            -loaded fatty acid-unsupplemented cells was found to be at least twice as great as that from Sr\n            <jats:sup>2+<\/jats:sup>\n            -loaded 18:2-enriched cells. The influence of enrichment with other fatty acids on Sr\n            <jats:sup>2+<\/jats:sup>\n            efflux was variable. The results reveal an enhanced Sr\n            <jats:sup>2+<\/jats:sup>\n            accumulation ability of\n            <jats:italic>S. cerevisiae<\/jats:italic>\n            following 18:2-enrichment, which is attributed to diminished Sr\n            <jats:sup>2+<\/jats:sup>\n            efflux activity in these cells.\n          <\/jats:p>","DOI":"10.1128\/aem.65.3.1191-1197.1999","type":"journal-article","created":{"date-parts":[[2019,12,19]],"date-time":"2019-12-19T19:48:15Z","timestamp":1576784895000},"page":"1191-1197","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":15,"title":["Stimulation of Strontium Accumulation in Linoleate-Enriched\n            <i>Saccharomyces cerevisiae<\/i>\n            Is a Result of Reduced Sr\n            <sup>2+<\/sup>\n            Efflux"],"prefix":"10.1128","volume":"65","author":[{"given":"Simon V.","family":"Avery","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Biology, University Plaza, Georgia State University, Atlanta, Georgia 30302-4010"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shareeka L.","family":"Smith","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Biology, University Plaza, Georgia State University, Atlanta, Georgia 30302-4010"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. Mohamad","family":"Ghazi","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Biology, University Plaza, Georgia State University, Atlanta, Georgia 30302-4010"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael J.","family":"Hoptroff","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Biology, University Plaza, Georgia State University, Atlanta, Georgia 30302-4010"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1099\/13500872-142-3-469"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(98)01192-X"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01569888"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.58.12.3883-3889.1992"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.62.11.3960-3966.1996"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1139\/o59-099"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0302-4598(86)80073-3"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.8.13.7926378"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(97)00446-8"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.196.1.157"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1177\/10454411930040032201"},{"key":"e_1_3_2_13_2","first-page":"1063","article-title":"Low concentrations of trifluoperazine arrest the cell division cycle of Saccharomyces cerevisiae at two specific stages.","volume":"134","author":"Eilam Y.","year":"1988","unstructured":"Eilam Y. 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