{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T02:58:36Z","timestamp":1773629916436,"version":"3.50.1"},"reference-count":36,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[1999,3,1]],"date-time":"1999-03-01T00:00:00Z","timestamp":920246400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[1999,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Aiming to develop a DNA marker specific for\n            <jats:italic>Bacillus anthracis<\/jats:italic>\n            and able to discriminate this species from\n            <jats:italic>Bacillus cereus<\/jats:italic>\n            ,\n            <jats:italic>Bacillus thuringiensis<\/jats:italic>\n            , and\n            <jats:italic>Bacillus mycoides<\/jats:italic>\n            , we applied the randomly amplified polymorphic DNA (RAPD) fingerprinting technique to a collection of 101 strains of the genus\n            <jats:italic>Bacillus<\/jats:italic>\n            , including 61 strains of the\n            <jats:italic>B. cereus<\/jats:italic>\n            group. An 838-bp RAPD marker (SG-850) specific for\n            <jats:italic>B. cereus<\/jats:italic>\n            ,\n            <jats:italic>B. thuringiensis<\/jats:italic>\n            ,\n            <jats:italic>B. anthracis<\/jats:italic>\n            , and\n            <jats:italic>B. mycoides<\/jats:italic>\n            was identified. This fragment included a putative (366-nucleotide) open reading frame highly homologous to the\n            <jats:italic>ypuA<\/jats:italic>\n            gene of\n            <jats:italic>Bacillus subtilis<\/jats:italic>\n            . The restriction analysis of the SG-850 fragment with\n            <jats:italic>Alu<\/jats:italic>\n            I distinguished\n            <jats:italic>B. anthracis<\/jats:italic>\n            from the other species of the\n            <jats:italic>B. cereus<\/jats:italic>\n            group.\n          <\/jats:p>","DOI":"10.1128\/aem.65.3.1298-1303.1999","type":"journal-article","created":{"date-parts":[[2019,12,19]],"date-time":"2019-12-19T19:48:28Z","timestamp":1576784908000},"page":"1298-1303","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":66,"title":["A Randomly Amplified Polymorphic DNA Marker Specific for the\n            <i>Bacillus cereus<\/i>\n            Group Is Diagnostic for\n            <i>Bacillus anthracis<\/i>"],"prefix":"10.1128","volume":"65","author":[{"given":"Daniele","family":"Daffonchio","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, Universita\u0300 degli Studi di Milano, 20133 Milan,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara","family":"Borin","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, Universita\u0300 degli Studi di Milano, 20133 Milan,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giuseppe","family":"Frova","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, Universita\u0300 degli Studi di Milano, 20133 Milan,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Romina","family":"Gallo","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Dipartimento di Biologia Animale e Genetica, Universita\u0300 degli Studi, 50125 Florence,2 Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"Mori","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Dipartimento di Biologia Animale e Genetica, Universita\u0300 degli Studi, 50125 Florence,2 Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renato","family":"Fani","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Dipartimento di Biologia Animale e Genetica, Universita\u0300 degli Studi, 50125 Florence,2 Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia","family":"Sorlini","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, Universita\u0300 degli Studi di Milano, 20133 Milan,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.178.2.377-384.1996"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/173545a0"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1992.tb05292.x"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-41-3-343"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1472-765X.1991.tb00608.x"},{"key":"e_1_3_2_7_2","unstructured":"Bazzicalupo\nM.\nFani\nR.\nThe use of RAPD for generating specific DNA probes for microorganisms.\nMethods in molecular biology\nClapp\nJ. 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