{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T13:19:34Z","timestamp":1790860774462,"version":"4.1.0"},"reference-count":28,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[1999,6,1]],"date-time":"1999-06-01T00:00:00Z","timestamp":928195200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[1999,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Production of phenazine antibiotics by the biological control bacterium\n            <jats:italic>Pseudomonas aureofaciens<\/jats:italic>\n            30-84 is regulated in part by the PhzI\/PhzR\n            <jats:italic>N<\/jats:italic>\n            -acyl-homoserine lactone (AHL) response system (L. S. Pierson III, V. D. Keppenne, and D. W. Wood, J. Bacteriol. 176:3966\u20133974, 1994; D. W. Wood and L. S. Pierson III, Gene 168:49\u201353, 1996). Two mutants, 30-84W and 30-84.A2, were isolated and were found to be deficient in the production of phenazine, protease, hydrogen cyanide (HCN), and the AHL signal\n            <jats:italic>N<\/jats:italic>\n            -hexanoyl-homoserine lactone. These mutants were not complemented by\n            <jats:italic>phzI<\/jats:italic>\n            ,\n            <jats:italic>phzR<\/jats:italic>\n            , or the phenazine biosynthetic genes (\n            <jats:italic>phzFABCD<\/jats:italic>\n            ) (L. S. Pierson III, T. Gaffney, S. Lam, and F. Gong, FEMS Microbiol. Lett. 134:299\u2013307, 1995). A 2.2-kb region of the 30-84 chromosome which fully restored production of all of these compounds in strain 30-84W was identified. Nucleotide sequence analysis of this region revealed a single open reading frame encoding a predicted 213-amino-acid protein which is very similar to the global response regulator GacA. Strain 30-84.A2 was not complemented by\n            <jats:italic>gacA<\/jats:italic>\n            or any cosmid from a genomic library of strain 30-84 but was complemented by\n            <jats:italic>gacS<\/jats:italic>\n            (formerly\n            <jats:italic>lemA<\/jats:italic>\n            ) homologs from\n            <jats:italic>Pseudomonas fluorescens<\/jats:italic>\n            Pf-5 (N. Corbel and J. E. Loper, J. Bacteriol. 177:6230\u20136236, 1995) and\n            <jats:italic>Pseudomonas syringae<\/jats:italic>\n            pv. syringae B728a (E. M. Hrabek and D. K. Willis, J. Bacteriol. 174:3011\u20133020, 1992). Transcription of\n            <jats:italic>phzR<\/jats:italic>\n            was not altered in either mutant; however,\n            <jats:italic>phzI<\/jats:italic>\n            transcription was eliminated in strains 30-84W and 30-84.A2. These results indicated that the GacS\/GacA two-component signal transduction system of\n            <jats:italic>P. aureofaciens<\/jats:italic>\n            30-84 controls the production of AHL required for phenazine production by mediating the transcription of\n            <jats:italic>phzI<\/jats:italic>\n            . Addition of exogenous AHL did not complement either mutant for phenazine production, indicating that the GacS\/GacA global regulatory system controls phenazine production at multiple levels. Our results reveal for the first time a mechanism by which a two-component regulatory system and an AHL-mediated regulatory system interact.\n          <\/jats:p>","DOI":"10.1128\/aem.65.6.2294-2299.1999","type":"journal-article","created":{"date-parts":[[2019,12,19]],"date-time":"2019-12-19T19:51:40Z","timestamp":1576785100000},"page":"2294-2299","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":144,"title":["Two-Component Transcriptional Regulation of N -Acyl-Homoserine Lactone Production in\n            <i>Pseudomonas aureofaciens<\/i>"],"prefix":"10.1128","volume":"65","author":[{"given":"S. T.","family":"Chancey","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Plant Pathology, University of Arizona, Tucson, Arizona 85721,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. W.","family":"Wood","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Department of Microbiology, University of Washington, Seattle, Washington 981952"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"III","given":"L. S.","family":"Pierson","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Plant Pathology, University of Arizona, Tucson, Arizona 85721,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.177.21.6230-6236.1995"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.77.12.7347"},{"key":"e_1_3_2_4_2","unstructured":"Dunlap\nP. V.\nN -Acyl- l -homoserine lactone autoinducers in bacteria: unity and diversity\nBacteria as multicellular organisms.\nShapiro\nJ. A.\nDworkin\nM.\n1996\n69\n106\nOxford University Press\nLondon United Kingdom"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(87)90041-2"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.176.2.269-275.1994"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.174.9.3011-3020.1992"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1998.00842.x"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1996.00063.x"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.5.1562"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(93)90691-U"},{"key":"e_1_3_2_12_2","unstructured":"Miller\nJ. H.\nExperiments in molecular genetics\n1972\n352\n355\nCold Spring Harbor Laboratory\nCold Spring Harbor N.Y"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ge.26.120192.000443"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI.1998.11.11.1078"},{"key":"e_1_3_2_15_2","doi-asserted-by":"crossref","unstructured":"Pierson\nL. S.\nIII\nChancey\nS. T.\nWood\nD. W.\nPhenazine antibiotic biosynthesis in the biological control bacterium Pseudomonas aureofaciens 30-84 is regulated at multiple levels\nAdvances in molecular genetics of plant-microbe interactions.\nStacy\nG.\nGresshoff\nP.\n1996\n463\n468\nKluwer Academic Publishers\nBoston Mass","DOI":"10.1016\/0378-1097(95)00423-X"},{"key":"e_1_3_2_16_2","first-page":"299","article-title":"Molecular analysis of genes encoding phenazine biosynthesis in the biological control bacterium Pseudomonas aureofaciens 30-84.","volume":"134","author":"Pierson L. 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