{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T03:56:03Z","timestamp":1789185363717,"version":"build-2803163510"},"reference-count":31,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[1999,7,1]],"date-time":"1999-07-01T00:00:00Z","timestamp":930787200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[1999,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            \u201c\n            <jats:italic>Dehalococcoides ethenogenes<\/jats:italic>\n            \u201d 195 can reductively dechlorinate tetrachloroethene (PCE) completely to ethene (ETH). When PCE-grown strain 195 was transferred (2% [vol\/vol] inoculum) into growth medium amended with trichloroethene (TCE),\n            <jats:italic>cis<\/jats:italic>\n            -dichloroethene (DCE), 1,1-DCE, or 1,2-dichloroethane (DCA) as an electron acceptor, these chlorinated compounds were consumed at increasing rates over time, which indicated that growth occurred. Moreover, the number of cells increased when TCE, 1,1-DCE, or DCA was present. PCE, TCE, 1,1-DCE, and\n            <jats:italic>cis<\/jats:italic>\n            -DCE were converted mainly to vinyl chloride (VC) and then to ETH, while DCA was converted to ca. 99% ETH and 1% VC.\n            <jats:italic>cis<\/jats:italic>\n            -DCE was used at lower rates than PCE, TCE, 1,1-DCE, or DCA was used. When PCE-grown cultures were transferred to media containing VC or\n            <jats:italic>trans<\/jats:italic>\n            -DCE, products accumulated slowly, and there was no increase in the rate, which indicated that these two compounds did not support growth. When the intermediates in PCE dechlorination by strain 195 were monitored, TCE was detected first, followed by\n            <jats:italic>cis<\/jats:italic>\n            -DCE. After a lag, VC, 1,1-DCE, and\n            <jats:italic>trans<\/jats:italic>\n            -DCE accumulated, which is consistent with the hypothesis that\n            <jats:italic>cis<\/jats:italic>\n            -DCE is the precursor of these compounds. Both\n            <jats:italic>cis<\/jats:italic>\n            -DCE and 1,1-DCE were eventually consumed, and both of these compounds could be considered intermediates in PCE dechlorination, whereas the small amount of\n            <jats:italic>trans<\/jats:italic>\n            -DCE that was produced persisted. Cultures grown on TCE, 1,1-DCE, or DCA could immediately dechlorinate PCE, which indicated that PCE reductive dehalogenase activity was constitutive when these electron acceptors were used.\n          <\/jats:p>","DOI":"10.1128\/aem.65.7.3108-3113.1999","type":"journal-article","created":{"date-parts":[[2019,12,19]],"date-time":"2019-12-19T19:53:07Z","timestamp":1576785187000},"page":"3108-3113","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":288,"title":["Reductive Dechlorination of Chlorinated Ethenes and 1,2-Dichloroethane by \u201c\n            <i>Dehalococcoides ethenogenes<\/i>\n            \u201d 195"],"prefix":"10.1128","volume":"65","author":[{"given":"Xavier","family":"Maymo\u0301-Gatell","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Section of Microbiology, Cornell University, Ithaca, New York 14853-8101"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy","family":"Anguish","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Section of Microbiology, Cornell University, Ithaca, New York 14853-8101"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen H.","family":"Zinder","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Section of Microbiology, Cornell University, Ithaca, New York 14853-8101"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.56.8.2511-2516.1990"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.58.6.1996-2000.1992"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.58.11.3622-3629.1992"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.57.8.2287-2292.1991"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.mi.45.100191.001435"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.54.12.2976-2980.1988"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1988.tb02706.x"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1021\/es960756r"},{"key":"e_1_3_2_10_2","unstructured":"Fennell\nD. 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