{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T05:43:00Z","timestamp":1790833380415,"version":"4.1.0"},"reference-count":19,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[1999,7,1]],"date-time":"1999-07-01T00:00:00Z","timestamp":930787200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[1999,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>A direct immunofluorescence assay (DFA) (Merifluor; Meridian Diagnostics, Inc., Cincinnati, Ohio) was compared to an immunomagnetic separation (IMS) assay (Dynabeads; Dynal, Inc., Lake Success, N.Y.) coupled with immunofluorescent microscopy (Waterborne, Inc., New Orleans, La.) for their ability to detect low concentrations of<jats:italic>Cryptosporidium parvum<\/jats:italic>oocysts in adult bovine fecal material. IMS-DFA resulted in a 2-log-unit increase in sensitivity (10 oocysts\/g) compared to DFA alone (1,000 oocysts\/g). The higher sensitivity obtained with IMS-DFA resulted from testing 2 g of fecal material instead of the 13 to 19 mg of fecal material tested in the DFA; the increased sensitivity was not attributable to a higher percent recovery.<\/jats:p>","DOI":"10.1128\/aem.65.7.3236-3239.1999","type":"journal-article","created":{"date-parts":[[2019,12,19]],"date-time":"2019-12-19T19:53:24Z","timestamp":1576785204000},"page":"3236-3239","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":37,"title":["Comparison of Sensitivity of Immunofluorescent Microscopy to That of a Combination of Immunofluorescent Microscopy and Immunomagnetic Separation for Detection of<i>Cryptosporidium parvum<\/i>Oocysts in Adult Bovine Feces"],"prefix":"10.1128","volume":"65","author":[{"given":"Maria das Grac\u0327as C.","family":"Pereira","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Veterinary Medicine Teaching and Research Center, School of Veterinary Medicine, University of California, Davis, Tulare, California 93274"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward R.","family":"Atwill","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Veterinary Medicine Teaching and Research Center, School of Veterinary Medicine, University of California, Davis, Tulare, California 93274"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ted","family":"Jones","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Veterinary Medicine Teaching and Research Center, School of Veterinary Medicine, University of California, Davis, Tulare, California 93274"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.2307\/3282084"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.63.10.3946-3949.1997"},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","first-page":"1116","DOI":"10.2460\/ajvr.1998.59.09.1116","article-title":"Evaluation of periparturient dairy cows and contact surfaces as a reservoir of Cryptosporidium parvum for calfhood infection.","volume":"59","author":"Atwill E. R.","year":"1998","unstructured":"Atwill E. R. Harp J. A. Jones T. Jardon P. W. Checel S. Zylstra M. Evaluation of periparturient dairy cows and contact surfaces as a reservoir of Cryptosporidium parvum for calfhood infection. Am. J. Vet. Res. 59 1998 1116 1121","journal-title":"Am. J. Vet. Res."},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"420","DOI":"10.2460\/ajvr.1999.60.04.420","article-title":"Age, geographic, and temporal distribution of fecal shedding of Cryptosporidium parvum oocysts in cow-calf herds.","volume":"60","author":"Atwill E. R.","year":"1999","unstructured":"Atwill E. R. Johnson E. Klingborg D. J. Veserat G. M. Markegard G. Jensen W. A. Pratt D. W. Delmas R. E. George H. A. Forero L. C. Philips R. L. Barry S. J. McDougald N. K. Gildersleeve R. R. Frost W. E. Age, geographic, and temporal distribution of fecal shedding of Cryptosporidium parvum oocysts in cow-calf herds. Am. J. Vet. Res. 60 1999 420 425","journal-title":"Am. J. Vet. Res."},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.64.11.4495-4499.1998"},{"key":"e_1_3_2_7_2","unstructured":"Casemore D. P. Wright S. E. Coop R. L. Cryptosporidiosis\u2014human and animal epidemiology. The general biology of Cryptosporidium Cryptosporidium and cryptosporidiosis. Fayer R. 1997 65 92 CRC Press Inc. Boca Raton Fla"},{"key":"e_1_3_2_8_2","unstructured":"Cytel Software Corp Egret reference manual revision 4. 1997 Cytel Software Corp. Cambridge Mass"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.63.8.3134-3138.1997"},{"key":"e_1_3_2_10_2","unstructured":"Jones T. Atwill E. R. Statistical methods for quantifying and comparing loading of Cryptosporidium on watersheds from different livestock sources using epidemiological survey data Proceedings of the Source Water Assessment and Protection Conference 1998. 1998 201 208 National Water Research Institute Dallas Tex"},{"key":"e_1_3_2_11_2","first-page":"502","article-title":"Cryptosporidium infection in cats: prevalence of infection in domestic and feral cats in the Glasgow area.","volume":"129","author":"Mtambo M. M. A.","year":"1991","unstructured":"Mtambo M. M. A. Nash A. S. Blewett D. A. Smith H. V. Wright S. Cryptosporidium infection in cats: prevalence of infection in domestic and feral cats in the Glasgow area. Vet. Rec. 129 1991 502 504","journal-title":"Vet. Rec."},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.65.2.841-845.1999"},{"key":"e_1_3_2_13_2","unstructured":"Vaughn T. A. Mammology. W. B. 1978 Saunders Company Philadelphia Pa"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.29.7.1323-1327.1991"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.30.11.2869-2873.1992"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268800058271"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4017(95)00811-X"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.31.11.2944-2946.1993"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-3306.1994.tb04323.x"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.2307\/3283345"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.65.7.3236-3239.1999","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.65.7.3236-3239.1999","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,28]],"date-time":"2024-07-28T23:04:01Z","timestamp":1722207841000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.65.7.3236-3239.1999"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,7]]},"references-count":19,"journal-issue":{"issue":"7","published-print":{"date-parts":[[1999,7]]}},"alternative-id":["10.1128\/AEM.65.7.3236-3239.1999"],"URL":"https:\/\/doi.org\/10.1128\/aem.65.7.3236-3239.1999","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[1999,7]]},"assertion":[{"value":"1998-10-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1999-04-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"1999-07-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}