{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T16:52:18Z","timestamp":1791305538190,"version":"4.3.1"},"reference-count":20,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[1999,7,1]],"date-time":"1999-07-01T00:00:00Z","timestamp":930787200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[1999,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A nested PCR was developed for detection of the\n            <jats:italic>Clostridium botulinum<\/jats:italic>\n            type C\n            <jats:sub>1<\/jats:sub>\n            toxin gene in sediments collected from wetlands where avian botulism outbreaks had or had not occurred. The C\n            <jats:sub>1<\/jats:sub>\n            toxin gene was detected in 16 of 18 sites, demonstrating both the ubiquitous distribution of\n            <jats:italic>C. botulinum<\/jats:italic>\n            type C in wetland sediments and the sensitivity of the detection assay.\n          <\/jats:p>","DOI":"10.1128\/aem.65.7.3240-3243.1999","type":"journal-article","created":{"date-parts":[[2019,12,19]],"date-time":"2019-12-19T19:54:13Z","timestamp":1576785253000},"page":"3240-3243","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":35,"title":["In Situ Detection of the\n            <i>Clostridium botulinum<\/i>\n            Type C\n            <sub>1<\/sub>\n            Toxin Gene in Wetland Sediments with a Nested PCR Assay"],"prefix":"10.1128","volume":"65","author":[{"given":"Judy L.","family":"Williamson","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->U.S. Fish and Wildlife Service, National Wildlife Health Center, Madison, Wisconsin 53711,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tonie E.","family":"Rocke","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->U.S. Fish and Wildlife Service, National Wildlife Health Center, Madison, Wisconsin 53711,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judd M.","family":"Aiken","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Department of Animal Health and Biomedical Sciences, University of Wisconsin\u2014Madison, Madison, Wisconsin 537062"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.59.5.1318-1324.1993"},{"key":"e_1_3_2_3_2","unstructured":"Ausubel\nF. 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