{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T03:29:26Z","timestamp":1789615766317,"version":"build-2803163510"},"reference-count":13,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[1999,8,1]],"date-time":"1999-08-01T00:00:00Z","timestamp":933465600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[1999,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Different methods of extraction of bacterial DNA from bovine milk to improve the direct detection of\n            <jats:italic>Brucella<\/jats:italic>\n            by PCR were evaluated. We found that the use of a lysis buffer with high concentrations of Tris, EDTA, and NaCl, high concentrations of sodium dodecyl sulfate and proteinase K, and high temperatures of incubation was necessary for the efficient extraction of\n            <jats:italic>Brucella<\/jats:italic>\n            DNA. The limit of detection by PCR was 5 to 50\n            <jats:italic>Brucella<\/jats:italic>\n            CFU\/ml of milk.\n          <\/jats:p>","DOI":"10.1128\/aem.65.8.3735-3737.1999","type":"journal-article","created":{"date-parts":[[2019,12,19]],"date-time":"2019-12-19T19:54:28Z","timestamp":1576785268000},"page":"3735-3737","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":55,"title":["Improved Method for Purification of Bacterial DNA from Bovine Milk for Detection of\n            <i>Brucella<\/i>\n            spp. by PCR"],"prefix":"10.1128","volume":"65","author":[{"given":"C.","family":"Romero","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Departamento de Microbiolog\u0131\u0301a, Universidad de Navarra, 31008 Pamplona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Lopez-Gon\u0303i","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Departamento de Microbiolog\u0131\u0301a, Universidad de Navarra, 31008 Pamplona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","unstructured":"Alton\nG. 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A.\nStruhl\nK.\n1990\n241\n245\nGreene Publishing Associates Inc. and John Wiley & Sons Inc.\nNew York N.Y"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.65.8.3735-3737.1999","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.65.8.3735-3737.1999","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T08:55:40Z","timestamp":1645606540000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.65.8.3735-3737.1999"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,8]]},"references-count":13,"journal-issue":{"issue":"8","published-print":{"date-parts":[[1999,8]]}},"alternative-id":["10.1128\/AEM.65.8.3735-3737.1999"],"URL":"https:\/\/doi.org\/10.1128\/aem.65.8.3735-3737.1999","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[1999,8]]},"assertion":[{"value":"1999-02-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1999-05-09","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"1999-08-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}