{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:32:13Z","timestamp":1787016733007,"version":"build-2736575974"},"reference-count":27,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2000,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Prior to gene transfer experiments performed with nonsterile soil, plasmid pJP4 was introduced into a donor microorganism,\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    ATCC 15224, by plate mating with\n                    <jats:italic>Ralstonia eutropha<\/jats:italic>\n                    JMP134. Genes on this plasmid encode mercury resistance and partial 2,4-dichlorophenoxyacetic acid (2,4-D) degradation. The\n                    <jats:italic>E. coli<\/jats:italic>\n                    donor lacks the chromosomal genes necessary for mineralization of 2,4-D, and this fact allows presumptive transconjugants obtained in gene transfer studies to be selected by plating on media containing 2,4-D as the carbon source. Use of this donor counterselection approach enabled detection of plasmid pJP4 transfer to indigenous populations in soils and under conditions where it had previously not been detected. In Madera Canyon soil, the sizes of the populations of presumptive indigenous transconjugants were 10\n                    <jats:sup>7<\/jats:sup>\n                    and 10\n                    <jats:sup>8<\/jats:sup>\n                    transconjugants g of dry soil\n                    <jats:sup>\u22121<\/jats:sup>\n                    for samples supplemented with 500 and 1,000 \u03bcg of 2,4-D g of dry soil\n                    <jats:sup>\u22121<\/jats:sup>\n                    , respectively. Enterobacterial repetitive intergenic consensus PCR analysis of transconjugants resulted in diverse molecular fingerprints. Biolog analysis showed that all of the transconjugants were members of the genus\n                    <jats:italic>Burkholderia<\/jats:italic>\n                    or the genus\n                    <jats:italic>Pseudomonas<\/jats:italic>\n                    . No mercury-resistant, 2,4-D-degrading microorganisms containing large plasmids or the\n                    <jats:italic>tfdB<\/jats:italic>\n                    gene were found in 2,4-D-amended uninoculated control microcosms. Thus, all of the 2,4-D-degrading isolates that contained a plasmid whose size was similar to the size of pJP4, contained the\n                    <jats:italic>tfdB<\/jats:italic>\n                    gene, and exhibited mercury resistance were considered transconjugants. In addition, slightly enhanced rates of 2,4-D degradation were observed at distinct times in soil that supported transconjugant populations compared to controls in which no gene transfer was detected.\n                  <\/jats:p>","DOI":"10.1128\/aem.66.1.290-296.2000","type":"journal-article","created":{"date-parts":[[2009,4,22]],"date-time":"2009-04-22T01:08:26Z","timestamp":1240362506000},"page":"290-296","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":61,"title":["Detection and Characterization of Plasmid pJP4 Transfer to Indigenous Soil Bacteria"],"prefix":"10.1128","volume":"66","author":[{"given":"D. T.","family":"Newby","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Microbiology and Immunology1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. L.","family":"Josephson","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Department of Soil, Water, and Environmental Science,2 University of Arizona, Tucson, Arizona 85721"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I. L.","family":"Pepper","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Microbiology and Immunology1 and"},{"name":"<!--label omitted: 2-->Department of Soil, Water, and Environmental Science,2 University of Arizona, Tucson, Arizona 85721"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0038-0717(93)90117-T"},{"key":"e_1_3_2_3_2","unstructured":"Chapman\nH.\nPratt\nP.\nMethods of analysis for soils plants and waters\n1961\n44\n46\nUniversity of California\nRiverside"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.62.2.515-521.1996"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.58.7.2180-2187.1992"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0168-6496(94)90027-2"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0168-6496(94)90008-6"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.62.7.2521-2526.1996"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.145.2.681-686.1981"},{"key":"e_1_3_2_10_2","first-page":"1012","article-title":"Fitnesses of a conjugative plasmid and its host bacteria in soil microcosms.","volume":"12","author":"Duncan K.","year":"1995","unstructured":"Duncan K. Fitnesses of a conjugative plasmid and its host bacteria in soil microcosms. Mol. Biol. Evol. 12 1995 1012 1021","journal-title":"Mol. Biol. Evol."},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00407938"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.228.4696.135"},{"key":"e_1_3_2_13_2","doi-asserted-by":"crossref","unstructured":"Ghosal\nD.\nYou\nI. S.\nChatterjee\nD. K.\nChakrabarty\nA. M.\nPlasmids in the degradation of chlorinated aromatic compounds\nPlasmids in bacteria.\nHelinski\nD. R.\n1985\n667\n686\nPlenum Press\nNew York N.Y","DOI":"10.1007\/978-1-4613-2447-8_47"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.59.6.1762-1766.1993"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00360881"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.60.11.4053-4058.1994"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6941.1992.tb01665.x"},{"key":"e_1_3_2_18_2","unstructured":"Roane\nT. M.\nBioaugmentation with metal-resistant microorganisms in the remediation of metal and organic contaminated soils. Ph.D. dissertation.\n1999\nUniversity of Arizona\nTucson"},{"key":"e_1_3_2_19_2","unstructured":"Rodriguez\nR. L.\nTait\nR. C.\nRecombinant DNA techniques: an introduction\n1983\n160\n162\nThe Benjamin-Cummings Publishing Co. Inc.\nMenlo Park Calif"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02091834"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.57.12.3482-3488.1991"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.61.5.1691-1698.1995"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.56.8.2471-2479.1990"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1000663619522"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0723-2020(89)80065-7"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/19.24.6823"},{"key":"e_1_3_2_27_2","first-page":"25","article-title":"Genomic fingerprinting of bacteria using repetitive sequence-based polymerase chain reaction.","volume":"5","author":"Versalovic J.","year":"1994","unstructured":"Versalovic J. Schneider M. de Bruijn F. J. Lupski J. R. Genomic fingerprinting of bacteria using repetitive sequence-based polymerase chain reaction. Methods Mol. Cell. Biol. 5 1994 25 40","journal-title":"Methods Mol. Cell. Biol."},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01570883"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.66.1.290-296.2000","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.66.1.290-296.2000","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T03:59:05Z","timestamp":1645588745000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.66.1.290-296.2000"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,1]]},"references-count":27,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2000,1]]}},"alternative-id":["10.1128\/AEM.66.1.290-296.2000"],"URL":"https:\/\/doi.org\/10.1128\/aem.66.1.290-296.2000","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,1]]},"assertion":[{"value":"1999-08-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1999-10-20","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2000-01-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}