{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T02:15:34Z","timestamp":1791252934825,"version":"4.1.0"},"reference-count":33,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2000,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Cryptosporidium parvum<\/jats:italic>\n            oocysts were stained with the fluorogenic dyes SYTO-9 and SYTO-59 and sorted by flow cytometry in order to determine whether the fluorescent staining intensity correlated with the ability of oocysts to infect neonatal CD-1 mice. Oocysts that did not fluoresce or that displayed weak fluorescent intensity when stained with SYTO-9 or SYTO-59 readily established infections in mice, whereas those oocysts that fluoresced brightly did not. Although fluorescent staining profiles varied among different batches of oocysts, a relative cutoff in fluorescent staining intensity that correlated with animal infectivity was observed for all batches.\n          <\/jats:p>","DOI":"10.1128\/aem.66.1.406-412.2000","type":"journal-article","created":{"date-parts":[[2009,4,22]],"date-time":"2009-04-22T05:08:26Z","timestamp":1240376906000},"page":"406-412","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":51,"title":["Comparison of Animal Infectivity and Nucleic Acid Staining for Assessment of\n            <i>Cryptosporidium parvum<\/i>\n            Viability in Water"],"prefix":"10.1128","volume":"66","author":[{"given":"Norman F.","family":"Neumann","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Biological Sciences,1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lyndon L.","family":"Gyu\u0308rek","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Biological Sciences,1"},{"name":"<!--label omitted: 2-->Department of Civil and Environmental Engineering,2 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leslie","family":"Gammie","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->EPCOR,3 Edmonton, Alberta T6G 2E9, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gordon R.","family":"Finch","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Department of Civil and Environmental Engineering,2 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miodrag","family":"Belosevic","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Biological Sciences,1"},{"name":"<!--label omitted: 4-->Medical Microbiology and Immunology,4 University of Alberta, and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1472-765X.1990.tb00180.x"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(83)90100-5"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/147.2.222"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(97)00033-7"},{"key":"e_1_3_2_6_2","unstructured":"Belosevic\nM.\nTaghi-Kilani\nR.\nGuy\nR. 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