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ITS-PCR resulted in single fragments of 350 and 650 bp, respectively, from eight strains of<jats:italic>Yarrowia lipolytica<\/jats:italic>and seven strains of<jats:italic>Candida zeylanoides<\/jats:italic>. Digestion of amplicons with<jats:italic>Hin<\/jats:italic>fI and<jats:italic>Hae<\/jats:italic>III produced two fragments of 200 and 150 bp from<jats:italic>Y. lipolytica<\/jats:italic>and three fragments of 350, 150, and 100 bp from<jats:italic>C. zeylanoides<\/jats:italic>, respectively. Although these fragments showed species-specific patterns and confirmed species identification, characterization did not enable intraspecies typing. Contour-clamped heterogeneous electric field PFGE separated chromosomal DNA of<jats:italic>Y. lipolytica<\/jats:italic>into three to five bands, most larger than 2 Mbp, whereas six to eight bands in the range of 750 to 2,200 bp were obtained from<jats:italic>C. zeylanoides<\/jats:italic>. Karyotypes of both yeasts showed different polymorphic patterns among strains. RAPD analysis, using enterobacterial repetitive intergenic sequences as primers, discriminated between strains within the same species. Cluster analysis of patterns formed groups that correlated with the source of isolation. For ITS-PCR, extraction of DNA by boiling yeast cells was successfully used.<\/jats:p>","DOI":"10.1128\/aem.66.10.4340-4344.2000","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:55:30Z","timestamp":1027763730000},"page":"4340-4344","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":38,"title":["Molecular Characterization of<i>Yarrowia lipolytica<\/i>and<i>Candida zeylanoides<\/i>Isolated from Poultry"],"prefix":"10.1128","volume":"66","author":[{"given":"T.","family":"Deak","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Center for Food Safety and Quality Enhancement, University of Georgia, Griffin, Georgia 30223-1797,1 and"},{"name":"<!--label omitted: 2-->Department of Microbiology and Biotechnology, University of Horticulture and Food Science, Somloi ut 14-16, Budapest H-1118, Hungary2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Chen","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Center for Food Safety and Quality Enhancement, University of Georgia, Griffin, Georgia 30223-1797,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. 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