{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T02:32:35Z","timestamp":1790735555671,"version":"4.1.0"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2000,11,1]],"date-time":"2000-11-01T00:00:00Z","timestamp":973036800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2000,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    An iron-poor stream of nearly neutral pH polluted by mine tailings has been investigated for a natural phenomenon responsible for the polishing of heavy metals in mine wastewaters. A white mineralized mat, which was determined to be hydrozincite [Zn\n                    <jats:sub>5<\/jats:sub>\n                    (CO\n                    <jats:sub>3<\/jats:sub>\n                    )\n                    <jats:sub>2<\/jats:sub>\n                    (OH)\n                    <jats:sub>6<\/jats:sub>\n                    ] by X-ray diffraction analysis, was observed in the stream sediments mainly in spring. The precipitate shows a total organic matter residue of 10% dry weight and contains high concentrations of Pb, Cd, Ni, Cu, and other metals. Scanning electron microscopy analysis suggests that hydrozincite is mainly of biological origin. Dormant photosynthetic microorganisms have been retrieved from 1-year-old dry hydrozincite. The autofluorescent microorganisms were imaged by a scanning confocal laser microscope. A photosynthetic filamentous bacterium, classified as\n                    <jats:italic>Scytonema<\/jats:italic>\n                    sp. strain ING-1, was found associated with microalga\n                    <jats:italic>Chlorella<\/jats:italic>\n                    sp. strain SA1. This microbial community is responsible for the natural polishing of heavy metals in the water stream by coprecipitation with hydrozincite.\n                  <\/jats:p>","DOI":"10.1128\/aem.66.11.5092-5098.2000","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T05:55:30Z","timestamp":1027749330000},"page":"5092-5098","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":70,"title":["Heavy Metal Coprecipitation with Hydrozincite [Zn\n                    <sub>5<\/sub>\n                    (CO\n                    <sub>3<\/sub>\n                    )\n                    <sub>2<\/sub>\n                    (OH)\n                    <sub>6<\/sub>\n                    ] from Mine Waters Caused by Photosynthetic Microorganisms"],"prefix":"10.1128","volume":"66","author":[{"given":"Francesca","family":"Podda","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Earth Sciences, University of Cagliari, 09127 Cagliari,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Zuddas","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Earth Sciences, University of Cagliari, 09127 Cagliari,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Minacci","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Department of Environmental Sciences, University Ca\u0300Foscari, 30122 Venice,2 Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milva","family":"Pepi","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Department of Environmental Sciences, University Ca\u0300Foscari, 30122 Venice,2 Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franco","family":"Baldi","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Department of Environmental Sciences, University Ca\u0300Foscari, 30122 Venice,2 Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","unstructured":"Baldi\nF.\nKukhar\nV. P.\nUlberg\nZ. R.\nBioconversion and removal of metals and radionuclides\nPerspectives in bioremediation.\nWild\nJ. R.\n1997\n75\n91\nKluver Academic Publishers\nAmsterdam The Netherlands","DOI":"10.1007\/978-94-011-5684-4_7"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.3354\/meps153045"},{"key":"e_1_3_2_4_2","unstructured":"Buurman\nP.\nvan Lagen\nB.\nVelthorst\nE. J.\nTotal or organic carbon and total nitrogen (elemental analyzer)\nManual for soil and water analysis.\nBuurman\nP.\nvan Lagen\nB.\nVelthorst\nE. J.\n1996\n11\n16\nBackhuys Publishers\nLeiden The Netherlands"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0013-7952(93)90090-Y"},{"key":"e_1_3_2_6_2","first-page":"797","article-title":"Abandoned mine sites: implications for water quality","volume":"2","author":"Caboi R.","year":"1996","unstructured":"Caboi R. Cidu R. Fanfani L. Zuddas P. 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