{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T02:10:53Z","timestamp":1785809453098,"version":"3.56.0"},"reference-count":25,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2000,2,1]],"date-time":"2000-02-01T00:00:00Z","timestamp":949363200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2000,2]]},"abstract":"ABSTRACT<\/jats:title>\n \n The yield coefficient (YC) of\n Pseudomonas<\/jats:italic>\n sp. strain DP-4, a 2,4-dichlorophenol (DCP)-degrading organism, was estimated from the number of CFU produced at the expense of 1 unit amount of DCP at low concentrations. At a low concentration of DCP, the YC can be overestimated in pure culture, because DP-4 assimilated not only DCP but also uncharacterized organic compounds contaminating a mineral salt medium. The concentration of these uncharacterized organic compounds was nutritionally equivalent to 0.7 \u03bcg of DCP-C ml\n \u22121<\/jats:sup>\n . A mixed culture with non-DCP-degrading organisms resulted in elimination of ca. 99.9% of the uncharacterized organic compounds, and then DP-4 assimilated only DCP as a substrate. In a mixed culture, DP-4 degraded an initial concentration of 0.1 to 10 \u03bcg of C ml of DCP\n \u22121<\/jats:sup>\n and the number of CFU of DP-4 increased. In the mixed culture, DCP at an initial concentration of 0.07 \u03bcg of C ml\n \u22121<\/jats:sup>\n was degraded. However, the number of CFU of DP-4 did not increase. DCP at an extremely low initial concentration of 0.01 \u03bcg of C ml\n \u22121<\/jats:sup>\n was not degraded in mixed culture even by a high density, 10\n 5<\/jats:sup>\n CFU ml\n \u22121<\/jats:sup>\n , of DP-4. When glucose was added to this mixed culture to a final concentration of 1 \u03bcg of C ml\n \u22121<\/jats:sup>\n , the initial concentration of 0.01 \u03bcg of C ml of DCP\n \u22121<\/jats:sup>\n was degraded. These results suggested that DP-4 required cosubstrates to degrade DCP at an extremely low initial concentration of 0.01 \u03bcg of C ml\n \u22121<\/jats:sup>\n . The YCs of DP-4 at the expense of DCP alone decreased discontinuously with the decrease of the initial concentration of DCP, i.e., 1.5, 0.19, or 0 CFU per pg of DCP-C when 0.7 to 10, 0.1 to 0.5, or 0.07 \u03bcg of C ml of DCP\n \u22121<\/jats:sup>\n was degraded, respectively. In this study, we developed a new method to eliminate uncharacterized organic compounds, and we estimated the YC of DP-4 at the expense of DCP as a sole source of carbon.\n <\/jats:p>","DOI":"10.1128\/aem.66.2.566-570.2000","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:03:59Z","timestamp":1027764239000},"page":"566-570","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":22,"title":["Estimation of the Yield Coefficient of\n Pseudomonas<\/i>\n sp. Strain DP-4 with a Low Substrate (2,4-Dichlorophenol [DCP]) Concentration in a Mineral Medium from Which Uncharacterized Organic Compounds Were Eliminated by a Non-DCP-Degrading Organism"],"prefix":"10.1128","volume":"66","author":[{"given":"Mitsunori","family":"Tarao","sequence":"first","affiliation":[{"name":"Laboratory of Environmental Microbiology, Department of Environmental and Natural Resource Science, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masayuki","family":"Seto","sequence":"additional","affiliation":[{"name":"Laboratory of Environmental Microbiology, Department of Environmental and Natural Resource Science, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00175076"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0045-6535(96)00237-8"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.1982.0056"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.173.3999.836"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01054972"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.46.6.1258-1262.1983"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.50.4.977-983.1985"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/br.36.2.146-155.1972"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1021\/es00083a003"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0043-1354(76)90140-8"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4615-8318-9_5"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00171893"},{"key":"e_1_3_1_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.49.4.822-827.1985"},{"key":"e_1_3_1_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.50.5.1132-1136.1985"},{"key":"e_1_3_1_16_2","first-page":"11","article-title":"Respiratory rate and survival of a bacterium (Pseudomonas aeruginosa) under starved condition.","volume":"14","author":"Seto M.","year":"1988","unstructured":"Seto M. 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