{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T06:37:03Z","timestamp":1792305423892,"version":"4.4.0"},"reference-count":63,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2000,3,1]],"date-time":"2000-03-01T00:00:00Z","timestamp":951868800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2000,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            It has recently been noted that a diversity of hyperthermophilic microorganisms have the ability to reduce Fe(III) with hydrogen as the electron donor, but the reduction of Fe(III) or other metals by these organisms has not been previously examined in detail. When\n            <jats:italic>Pyrobaculum islandicum<\/jats:italic>\n            was grown at 100\u00b0C in a medium with hydrogen as the electron donor and Fe(III)-citrate as the electron acceptor, the increase in cell numbers of\n            <jats:italic>P. islandicum<\/jats:italic>\n            per mole of Fe(III) reduced was found to be ca. 10-fold higher than previously reported. Poorly crystalline Fe(III) oxide could also serve as the electron acceptor for growth on hydrogen. The stoichiometry of hydrogen uptake and Fe(III) oxide reduction was consistent with the oxidation of 1 mol of hydrogen resulting in the reduction of 2 mol of Fe(III). The poorly crystalline Fe(III) oxide was reduced to extracellular magnetite.\n            <jats:italic>P. islandicum<\/jats:italic>\n            could not effectively reduce the crystalline Fe(III) oxide minerals goethite and hematite. In addition to using hydrogen as an electron donor for Fe(III) reduction,\n            <jats:italic>P. islandicum<\/jats:italic>\n            grew via Fe(III) reduction in media in which peptone and yeast extract served as potential electron donors. The closely related species\n            <jats:italic>P. aerophilum<\/jats:italic>\n            grew via Fe(III) reduction in a similar complex medium. Cell suspensions of\n            <jats:italic>P. islandicum<\/jats:italic>\n            reduced the following metals with hydrogen as the electron donor: U(VI), Tc(VII), Cr(VI), Co(III), and Mn(IV). The reduction of these metals was dependent upon the presence of cells and hydrogen. The metalloids arsenate and selenate were not reduced. U(VI) was reduced to the insoluble U(IV) mineral uraninite, which was extracellular. Tc(VII) was reduced to insoluble Tc(IV) or Tc(V). Cr(VI) was reduced to the less toxic, less soluble Cr(III). Co(III) was reduced to Co(II). Mn(IV) was reduced to Mn(II) with the formation of manganese carbonate. These results demonstrate that biological reduction may contribute to the speciation of metals in hydrothermal environments and could account for such phenomena as magnetite accumulation and the formation of uranium deposits at ca. 100\u00b0C. Reduction of toxic metals with hyperthermophilic microorganisms or their enzymes might be applied to the remediation of metal-contaminated waters or waste streams.\n          <\/jats:p>","DOI":"10.1128\/aem.66.3.1050-1056.2000","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:03:59Z","timestamp":1027764239000},"page":"1050-1056","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":248,"title":["Reduction of Fe(III), Mn(IV), and Toxic Metals at 100\u00b0C by\n            <i>Pyrobaculum islandicum<\/i>"],"prefix":"10.1128","volume":"66","author":[{"given":"Kazem","family":"Kashefi","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Microbiology, University of Massachusetts, Amherst, Massachusetts 01003"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Derek R.","family":"Lovley","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Microbiology, University of Massachusetts, Amherst, Massachusetts 01003"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"635","article-title":"Anaerobic reduction of ferric iron by hydrogen bacteria.","volume":"48","author":"Balashova V. 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