{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T02:39:53Z","timestamp":1789180793510,"version":"build-2803163510"},"reference-count":27,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2000,4,1]],"date-time":"2000-04-01T00:00:00Z","timestamp":954547200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2000,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Members of the genera<jats:italic>Desulfuromonas<\/jats:italic>and<jats:italic>Dehalococcoides<\/jats:italic>reductively dechlorinate tetrachloroethene (PCE) and trichloroethene. Two primer pairs specific to hypervariable regions of the 16S rRNA genes of the<jats:italic>Dehalococcoides<\/jats:italic>group (comprising<jats:italic>Dehalococcoides ethenogenes<\/jats:italic>and<jats:italic>Dehalococcoides<\/jats:italic>sp. strain FL2) and the acetate-oxidizing, PCE-dechlorinating<jats:italic>Desulfuromonas<\/jats:italic>group (comprising<jats:italic>Desulfuromonas<\/jats:italic>sp. strain BB1 and<jats:italic>Desulfuromonas chloroethenica<\/jats:italic>) were designed. The detection threshold of a nested PCR approach using universal bacterial primers followed by a second PCR with the<jats:italic>Desulfuromonas<\/jats:italic>dechlorinator-targeted primer pair was 1 \u00d7 10<jats:sup>3<\/jats:sup>BB1 cells added per gram (wet weight) of sandy aquifer material. Total community DNA isolated from sediments of three Michigan rivers and six different chloroethene-contaminated aquifer samples was used as template in nested PCR. All river sediment samples yielded positive signals with the BB1- and the<jats:italic>Dehalococcoides<\/jats:italic>-targeted primers. One chloroethene-contaminated aquifer tested positive with the<jats:italic>Dehalococcoides<\/jats:italic>-targeted primers, and another contaminated aquifer tested positive with the<jats:italic>Desulfuromonas<\/jats:italic>dechlorinator-targeted primer pair. Restriction fragment analysis of the amplicons could discriminate strain BB1 from other known<jats:italic>Desulfuromonas<\/jats:italic>species. Microcosm studies confirmed the presence of PCE-dechlorinating, acetate-oxidizing<jats:italic>Desulfuromonas<\/jats:italic>and hydrogenotrophic<jats:italic>Dehalococcoides<\/jats:italic>species in samples yielding positive PCR signals with the specific primers.<\/jats:p>","DOI":"10.1128\/aem.66.4.1369-1374.2000","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T05:55:30Z","timestamp":1027749330000},"page":"1369-1374","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":276,"title":["16S rRNA Gene-Based Detection of Tetrachloroethene-Dechlorinating<i>Desulfuromonas<\/i>and<i>Dehalococcoides<\/i>Species"],"prefix":"10.1128","volume":"66","author":[{"given":"Frank E.","family":"Lo\u0308ffler","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Center for Microbial Ecology, Michigan State University, East Lansing, Michigan 48824-1325,1and"},{"name":"<!--label omitted: 2-->School of Civil and Environmental Engineering, Georgia Institute of Technology, Atlanta, Georgia 30332-05122"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Sun","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Center for Microbial Ecology, Michigan State University, East Lansing, Michigan 48824-1325,1and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jieran","family":"Li","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Center for Microbial Ecology, Michigan State University, East Lansing, Michigan 48824-1325,1and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James M.","family":"Tiedje","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Center for Microbial Ecology, Michigan State University, East Lansing, Michigan 48824-1325,1and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2237418"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","unstructured":"Abrahamson K. 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