{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T15:32:34Z","timestamp":1791127954864,"version":"4.1.0"},"reference-count":22,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2000,4,1]],"date-time":"2000-04-01T00:00:00Z","timestamp":954547200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2000,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Purified CO dehydrogenase (CODH) from\n            <jats:italic>Clostridium thermoaceticum<\/jats:italic>\n            catalyzed the transformation of 2,4,6-trinitrotoluene (TNT). The intermediates and reduced products of TNT transformation were separated and appear to be identical to the compounds formed by\n            <jats:italic>C. acetobutylicum<\/jats:italic>\n            , namely, 2-hydroxylamino-4,6-dinitrotoluene (2HA46DNT), 4-hydroxylamino-2,6-dinitrotoluene (4HA26DNT), 2,4-dihydroxylamino-6-nitrotoluene (24DHANT), and the Bamberger rearrangement product of 2,4-dihydroxylamino-6-nitrotoluene. In the presence of saturating CO, CODH catalyzed the conversion of TNT to two monohydroxylamino derivatives (2HA46DNT and 4HA26DNT), with 4HA26DNT as the dominant isomer. These derivatives were then converted to 24DHANT, which slowly converted to the Bamberger rearrangement product. Apparent\n            <jats:italic>\n              K\n              <jats:sub>m<\/jats:sub>\n            <\/jats:italic>\n            and\n            <jats:italic>k<\/jats:italic>\n            <jats:sub>cat<\/jats:sub>\n            values of TNT reduction were 165 \u00b1 43 \u03bcM for TNT and 400 \u00b1 94 s\n            <jats:sup>\u22121<\/jats:sup>\n            , respectively. Cyanide, an inhibitor for the CO\/CO\n            <jats:sub>2<\/jats:sub>\n            oxidation\/reduction activity of CODH, inhibited the TNT degradation activity of CODH.\n          <\/jats:p>","DOI":"10.1128\/aem.66.4.1474-1478.2000","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:55:30Z","timestamp":1027763730000},"page":"1474-1478","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":61,"title":["2,4,6-Trinitrotoluene Reduction by Carbon Monoxide Dehydrogenase from\n            <i>Clostridium thermoaceticum<\/i>"],"prefix":"10.1128","volume":"66","author":[{"given":"Shouqin","family":"Huang","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Biochemistry and Cell Biology and the Institute of Biosciences and Bioengineering1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul A.","family":"Lindahl","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->The Departments of Chemistry and of Biochemistry and Biophysics, Texas A&amp;M University, College Station, Texas 778432"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuanyue","family":"Wang","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->Department of Environmental Science and Engineering,3 Rice University, Houston, Texas 77005-1892, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George N.","family":"Bennett","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Biochemistry and Cell Biology and the Institute of Biosciences and Bioengineering1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frederick B.","family":"Rudolph","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Biochemistry and Cell Biology and the Institute of Biosciences and Bioengineering1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph B.","family":"Hughes","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->Department of Environmental Science and Engineering,3 Rice University, Houston, Texas 77005-1892, and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00195a011"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi952902w"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(20)64294-6"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja9731480"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja9528386"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/etc.5620170301"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1021\/es970612s"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jim.2900328"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.31.6.949-958.1976"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00290917"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.3109\/10409239109114070"},{"key":"e_1_3_2_13_2","first-page":"A470","article-title":"The amino acid sequence and redox properties of the 8-iron ferredoxin II from Clostridium thermoaceticum.","volume":"5","author":"Ragsdale S. 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