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When M2M medium was amended with EDTA, sMMO activity was similar to that in NMS medium, indicating that EDTA-bound copper had lower bioavailability than pyrophosphate-bound copper. EDTA did not limit the association of copper with the cells; rather, copper was sequestered in a form which did not affect sMMO activity.<\/jats:p>","DOI":"10.1128\/aem.66.4.1730-1733.2000","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:03:59Z","timestamp":1027764239000},"page":"1730-1733","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":35,"title":["Effect of Copper Speciation on Whole-Cell Soluble Methane Monooxygenase Activity in<i>Methylosinus trichosporium<\/i>OB3b"],"prefix":"10.1128","volume":"66","author":[{"given":"John D.","family":"Morton","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Environmental and Water Resources Engineering, Department of Civil and Environmental Engineering, The University of Michigan, Ann Arbor, Michigan 48109-2125"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kim F.","family":"Hayes","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Environmental and Water Resources Engineering, Department of Civil and Environmental Engineering, The University of Michigan, Ann Arbor, Michigan 48109-2125"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeremy D.","family":"Semrau","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Environmental and Water Resources Engineering, Department of Civil and Environmental Engineering, The University of Michigan, Ann Arbor, Michigan 48109-2125"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1021\/es950212"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1997.tb10284.x"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00117048"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.14.3606-3613.1998"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/mr.60.2.439-471.1996"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02543874"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.64.3.1106-1114.1998"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.14.7957"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.57.1.7-14.1991"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/bit.260380412"},{"key":"e_1_3_2_12_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/BF00695208","article-title":"Optimization and maintenance of soluble methane monooxygenase activity in Methylosinus trichosporium OB3b.","volume":"5","author":"Sayler G. 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