{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T22:06:28Z","timestamp":1791065188796,"version":"4.1.0"},"reference-count":31,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2000,5,1]],"date-time":"2000-05-01T00:00:00Z","timestamp":957139200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2000,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Infective\n            <jats:italic>Cryptosporidium parvum<\/jats:italic>\n            oocysts were detected in mussels (\n            <jats:italic>Mytilus galloprovincialis<\/jats:italic>\n            ) and cockles (\n            <jats:italic>Cerastoderma edule<\/jats:italic>\n            ) from a shellfish-producing region (Gallaecia, northwest Spain, bounded by the Atlantic Ocean) that accounts for the majority of European shellfish production. Shellfish were collected from bay sites with different degrees of organic pollution. Shellfish harboring\n            <jats:italic>C. parvum<\/jats:italic>\n            oocysts were recovered only from areas located near the mouths of rivers with a high density of grazing ruminants on their banks. An approximation of the parasite load of shellfish collected in positive sites indicated that each shellfish transported more than 10\n            <jats:sup>3<\/jats:sup>\n            oocysts. Recovered oocysts were infectious for neonatal mice, and PCR-restriction fragment length polymorphism analysis demonstrated a profile similar to that described for genotype C or 2 of the parasite. These results demonstrate that mussels and cockles could act as a reservoir of\n            <jats:italic>C. parvum<\/jats:italic>\n            infection for humans. Moreover, estuarine shellfish could be used as an indicator of river water contamination.\n          <\/jats:p>","DOI":"10.1128\/aem.66.5.1866-1870.2000","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:55:30Z","timestamp":1027763730000},"page":"1866-1870","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":105,"title":["Detection of Infectious\n            <i>Cryptosporidium parvum<\/i>\n            Oocysts in Mussels (\n            <i>Mytilus galloprovincialis<\/i>\n            ) and Cockles (\n            <i>Cerastoderma edule<\/i>\n            )"],"prefix":"10.1128","volume":"66","author":[{"given":"M.","family":"Gomez-Bautista","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Parasitolog\u0131\u0301a y Enfermedades Parasitarias, Departamento de Patolog\u0131\u0301a Animal (Sanidad Animal),1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. M.","family":"Ortega-Mora","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Parasitolog\u0131\u0301a y Enfermedades Parasitarias, Departamento de Patolog\u0131\u0301a Animal (Sanidad Animal),1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Tabares","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Parasitolog\u0131\u0301a y Enfermedades Parasitarias, Departamento de Patolog\u0131\u0301a Animal (Sanidad Animal),1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Lopez-Rodas","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Gene\u0301tica, Departamento de Produccio\u0301n Animal,2Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Costas","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Gene\u0301tica, Departamento de Produccio\u0301n Animal,2Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"253","article-title":"La calidad de las aguas espa\u00f1olas.","volume":"177","author":"Alvarez Cobelas M.","year":"1997","unstructured":"Alvarez Cobelas M. Cabrera Capitan F. La calidad de las aguas espa\u00f1olas. Mundo Cient\u0131\u0301fico 177 1997 253 255","journal-title":"Mundo Cient\u0131\u0301fico"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1996.tb08107.x"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.28.3.495-503.1990"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.65.9.3958-3960.1997"},{"key":"e_1_3_2_6_2","unstructured":"Casemore\nD. P.\nWright\nS. E.\nCoop\nR. 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Macias F. Contaminaci\u00f3n org\u00e1nica de las r\u0131\u0301as gallegas. Cuadernos Marisqueros Publ. Tec. 12 1987 747 752","journal-title":"Cuadernos Marisqueros Publ. Tec."},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-308X(08)60117-7"},{"key":"e_1_3_2_18_2","unstructured":"Instituto Hidrogr\u00e1fico de la Marina\nDerrotero de la costa NW de Espa\u00f1a. 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