{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T15:41:49Z","timestamp":1792683709099,"version":"4.5.0"},"reference-count":27,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2000,5,1]],"date-time":"2000-05-01T00:00:00Z","timestamp":957139200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2000,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            For many years,\n            <jats:italic>Erysipelothrix rhusiopathiae<\/jats:italic>\n            has been known to be the causative agent of the occupationally related infection erysipeloid. A survey of the distribution of\n            <jats:italic>Erysipelothrix<\/jats:italic>\n            spp. in 19 Australasian seafoods was conducted, and methodologies for the detection of\n            <jats:italic>Erysipelothrix<\/jats:italic>\n            spp. were evaluated. Twenty-one\n            <jats:italic>Erysipelothrix<\/jats:italic>\n            spp. were isolated from 52 seafood parts. Primary isolation of\n            <jats:italic>Erysipelothrix<\/jats:italic>\n            spp. was most efficiently achieved with brain heart infusion broth enrichment followed by subculture onto a selective brain heart infusion agar containing kanamycin, neomycin, and vancomycin after 48 h of incubation. Selective tryptic soy broth, with 48 h of incubation, was the best culture method for the detection of\n            <jats:italic>Erysipelothrix<\/jats:italic>\n            spp. with PCR. PCR detection was 50% more sensitive than culture.\n            <jats:italic>E. rhusiopathiae<\/jats:italic>\n            was isolated from a variety of different fish, cephalopods, and crustaceans, including a Western rock lobster (\n            <jats:italic>Panulirus cygnus<\/jats:italic>\n            ). There was no significant correlation between the origin of the seafoods tested and the distribution of\n            <jats:italic>E. rhusiopathiae<\/jats:italic>\n            . An organism indistinguishable from\n            <jats:italic>Erysipelothrix tonsillarum<\/jats:italic>\n            was isolated for the first time from an Australian oyster and a silver bream. Overall,\n            <jats:italic>Erysipelothrix<\/jats:italic>\n            spp. were widely distributed in Australasian seafoods, illustrating the potential for erysipeloid-like infections in fishermen.\n          <\/jats:p>","DOI":"10.1128\/aem.66.5.2066-2070.2000","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:55:30Z","timestamp":1027763730000},"page":"2066-2070","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":37,"title":["Comparison of Methods for Detection of\n            <i>Erysipelothrix<\/i>\n            spp. and Their Distribution in Some Australasian Seafoods"],"prefix":"10.1128","volume":"66","author":[{"given":"S. G.","family":"Fidalgo","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Microbiology, The University of Western Australia,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Q.","family":"Wang","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Microbiology, The University of Western Australia,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. V.","family":"Riley","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Microbiology, The University of Western Australia,1 and"},{"name":"<!--label omitted: 2-->Division of Microbiology and Infectious Diseases,2 Western Australian Centre for Pathology and Medical Research, Queen Elizabeth II Medical Centre, Nedlands, Western Australia 6009, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"15.1.5","article-title":"The polymerase chain reaction","volume":"2","author":"Ausubel F. M.","year":"1992","unstructured":"Ausubel F. M. The polymerase chain reaction Current protocols in molecular biology Ausubel F. M. Brent R. Kingston R. E. Moore D. D. Seidman J. G. Smith J. A. Struhl K. 2 1992 15.1.5 John Wiley and Sons, Inc. Toronto, Canada","journal-title":"Current protocols in molecular biology"},{"key":"e_1_3_2_3_2","first-page":"4.4.4","article-title":"Preparation and analysis of RNA","volume":"1","author":"Ausubel F. M.","year":"1995","unstructured":"Ausubel F. M. Preparation and analysis of RNA Current protocols in molecular biology Ausubel F. M. Brent R. Kingston R. E. Moore D. D. Seidman J. G. Smith J. A. Struhl K. 1 1995 4.4.4 John Wiley and Sons, Inc. Toronto, Canada","journal-title":"Current protocols in molecular biology"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1186\/BF03547206"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.5694\/j.1326-5377.1999.tb127775.x"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-48-9-789"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.56.5.1448-1452.1990"},{"key":"e_1_3_2_8_2","unstructured":"Ewald\nF. W.\nThe genus Erysipelothrix The prokaryotes: a handbook on habitats isolation and identification of bacteria.\nStarr\nM. P.\nStolp\nH.\nTruper\nH. G.\nBalows\nA.\nSchlegel\nH. G.\n1981\n1688\n1700\nSpringer-Verlag\nNew York N.Y"},{"key":"e_1_3_2_9_2","first-page":"507","article-title":"Erysipeloid, with a record of 329 cases, of which 323 were caused by crab bites, or lesions produced by crabs.","volume":"22","author":"Gilchrist T. C.","year":"1904","unstructured":"Gilchrist T. C. Erysipeloid, with a record of 329 cases, of which 323 were caused by crab bites, or lesions produced by crabs. J. Cutan. Dis. 22 1904 507 519","journal-title":"J. Cutan. Dis."},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/10.2.317"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/am.22.1.141-142.1971"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(53)91807-8"},{"key":"e_1_3_2_13_2","unstructured":"Hunter\nD.\nThe diseases of occupations\n5th ed.\n1974\n709\n712\nThe English University Press Ltd.\nLondon England"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1938.02790410001001"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1605(98)00152-4"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.32.6.1526-1531.1994"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.46.4.343-349.1943"},{"key":"e_1_3_2_18_2","first-page":"169","article-title":"Isolation of Erysipelothrix rhusiopathiae from marine fish.","volume":"9","author":"Pestana de Castro A. F.","year":"1967","unstructured":"Pestana de Castro A. F. Campedelli Filho O. Troise C. Isolation of Erysipelothrix rhusiopathiae from marine fish. Rev. Inst. Med. Trop. Sao Paulo 9 1967 169 171","journal-title":"Rev. Inst. Med. Trop. Sao Paulo"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.2.4.354"},{"key":"e_1_3_2_20_2","unstructured":"Reboli\nA. C.\nFarrar\nW. E.\nThe genus Erysipelothrix The prokaryotes. A handbook on the biology of bacteria: ecophysiology isolation identification and applications.\nBalows\nA.\nTruper\nH. G.\nDworkin\nM.\nHarder\nW.\nSchleifer\nK.\n1992\n1629\n1642\nSpringer-Verlag\nNew York N.Y"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.5694\/j.1326-5377.1949.tb36913.x"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.1971.tb02291.x"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.1.86-89.1998"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-42-3-469"},{"key":"e_1_3_2_25_2","unstructured":"Tompkins\nL. S.\nMolecular epidemiology in infectious diseases\nInfectious diseases\n2nd ed.\nGorbach\nS. L.\nBartlett\nJ. G.\nBlacklow\nN. R.\n1998\n33\n34\nWB Saunders Co.\nPhiladelphia Pa"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.82.4.595-599.1961"},{"key":"e_1_3_2_27_2","unstructured":"Wood\nR. L. Erysipelothrix infection\nDiseases transmitted from animals to man\n6th ed.\nHubbert\nW. T.\nMcCullough\nW. F.\nSchnurrenberger\nP. R.\n1975\n271\n281\nThomas\nSpringfield Ill"},{"key":"e_1_3_2_28_2","first-page":"1303","article-title":"A selective liquid medium utilising antibiotics for isolation of Erysipelothrix insidiosa.","volume":"26","author":"Wood R. L.","year":"1965","unstructured":"Wood R. L. A selective liquid medium utilising antibiotics for isolation of Erysipelothrix insidiosa. Am. J. Vet. Res. 26 1965 1303 1308","journal-title":"Am. J. Vet. Res."}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.66.5.2066-2070.2000","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.66.5.2066-2070.2000","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T10:20:24Z","timestamp":1645611624000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.66.5.2066-2070.2000"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,5]]},"references-count":27,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2000,5]]}},"alternative-id":["10.1128\/AEM.66.5.2066-2070.2000"],"URL":"https:\/\/doi.org\/10.1128\/aem.66.5.2066-2070.2000","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,5]]},"assertion":[{"value":"1999-11-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2000-03-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2000-05-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}