{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T21:28:58Z","timestamp":1791408538468,"version":"4.3.3"},"reference-count":64,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2000,6,1]],"date-time":"2000-06-01T00:00:00Z","timestamp":959817600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2000,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The white-rot fungus\n            <jats:italic>Pleurotus ostreatus<\/jats:italic>\n            was able to degrade the polycyclic aromatic hydrocarbons (PAHs) benzo[\n            <jats:italic>a<\/jats:italic>\n            ]anthracene, chrysene, benzo[\n            <jats:italic>b<\/jats:italic>\n            ]fluoranthene, benzo[\n            <jats:italic>k<\/jats:italic>\n            ]fluoranthene, benzo[\n            <jats:italic>a<\/jats:italic>\n            ]pyrene, dibenzo[\n            <jats:italic>a<\/jats:italic>\n            ,\n            <jats:italic>h<\/jats:italic>\n            ]anthracene, and benzo[\n            <jats:italic>ghi<\/jats:italic>\n            ]perylene in nonsterile soil both in the presence and in the absence of cadmium and mercury. During 15 weeks of incubation, recovery of individual compounds was 16 to 69% in soil without additional metal. While soil microflora contributed mostly to degradation of pyrene (82%) and benzo[\n            <jats:italic>a<\/jats:italic>\n            ]anthracene (41%), the fungus enhanced the disappearance of less-soluble polycyclic aromatic compounds containing five or six aromatic rings. Although the heavy metals in the soil affected the activity of ligninolytic enzymes produced by the fungus (laccase and Mn-dependent peroxidase), no decrease in PAH degradation was found in soil containing Cd or Hg at 10 to 100 ppm. In the presence of cadmium at 500 ppm in soil, degradation of PAHs by soil microflora was not affected whereas the contribution of fungus was negligible, probably due to the absence of Mn-dependent peroxidase activity. In the presence of Hg at 50 to 100 ppm or Cd at 100 to 500 ppm, the extent of soil colonization by the fungus was limited.\n          <\/jats:p>","DOI":"10.1128\/aem.66.6.2471-2478.2000","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:55:30Z","timestamp":1027763730000},"page":"2471-2478","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":192,"title":["Influence of Cadmium and Mercury on Activities of Ligninolytic Enzymes and Degradation of Polycyclic Aromatic Hydrocarbons by\n            <i>Pleurotus ostreatus<\/i>\n            in Soil"],"prefix":"10.1128","volume":"66","author":[{"given":"Petr","family":"Baldrian","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Institute of Microbiology, Academy of Sciences of the Czech Republic, 14220 Prague 4, Czech Republic,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carsten","family":"in der Wiesche","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Institute of Plant Nutrition and Soil Science, FAL, 38116 Braunschweig, Germany2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jir\u030ci\u0301","family":"Gabriel","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Institute of Microbiology, Academy of Sciences of the Czech Republic, 14220 Prague 4, Czech Republic,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frantis\u030cek","family":"Nerud","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Institute of Microbiology, Academy of Sciences of the Czech Republic, 14220 Prague 4, Czech Republic,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frantis\u030cek","family":"Zadraz\u030cil","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Institute of Plant Nutrition and Soil Science, FAL, 38116 Braunschweig, Germany2"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","unstructured":"Anderson\nT.-H.\nDomsch\nK. H.\nEffects of soil structure on microbial biomass\nSoil structure\u2014its development and function.\nHartger\nK. H.\nStewart\nB. A.\n1995\n325\n345\nLewis Publishers\nBoca Raton Fla"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002530050875"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02826566"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02814716"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1021\/es00051a724"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0045-6535(93)90187-A"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-7992(93)90098-G"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.62.1.292-295.1996"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.62.7.2547-2553.1996"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.62.7.2554-2559.1996"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.62.5.1597-1603.1996"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2672.1999.00537.x"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.62.7.2381-2386.1996"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0038-0717(97)00219-8"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.173.13.4101-4106.1991"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3925550"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00369419"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1995.tb07504.x"},{"key":"e_1_3_2_20_2","unstructured":"Cerniglia\nC. E.\nHeitkamp\nM. A.\nMicrobial degradation of polycyclic aromatic hydrocarbons (PAH) in the aquatic environment\nMetabolism of polycyclic aromatic hydrocarbons in the aquatic environment.\nVaranasi\nU.\n1989\n41\n68\nCRC Press Inc.\nBoca Raton Fla"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.63.9.3444-3450.1997"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.62.12.4563-4567.1996"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.58.9.3000-3006.1992"},{"key":"e_1_3_2_24_2","unstructured":"Dipple\nA.\nCheng\nS. C.\nBigger\nC. A. H.\nPolycyclic aromatic hydrocarbon carcinogens\nMutagens and carcinogens in the diet.\nPariza\nM. W.\nAeschbacher\nH. U.\nFelton\nJ. S.\nSato\nS.\n1990\n109\n127\nWiley-Liss\nNew York N.Y"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/0141-0229(95)00109-3"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.120.1.450-457.1974"},{"key":"e_1_3_2_27_2","doi-asserted-by":"crossref","unstructured":"Giersig\nK.\nSchinner\nF.\nAbbau von polyzyklischen aromatischen Kohlenwasserstoffen in metallbelasteten Boden\nPraxis der mikrobiellen Bodensanierung.\nMargesin\nR.\nSchneider\nM.\nSchinner\nF.\n1995\n107\n117\nSpringer\nBerlin Germany","DOI":"10.1007\/978-3-642-85196-4_7"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(83)90672-1"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1008368923383"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002530050876"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.65.5.1834-1842.1999"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0048-9697(99)00253-3"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.64.8.2853-2858.1998"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.62.3.880-885.1996"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/0038-0717(94)90312-3"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002489900029"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02814664"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1998.tb13234.x"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1139\/m96-151"},{"key":"e_1_3_2_40_2","unstructured":"Loske\nD.\nH\u00fcttermann\nA.\nMajerczyk\nA.\nZadrazil\nF.\nLorsen\nH.\nWaldinger\nP.\nUse of white rot fungi for the clean-up of contaminated sites\nAdvances in biological treatment of lignocellulosic materials.\nCoughlan\nM. P.\nCollaco\nP.\n1990\n311\n321\nElsevier\nLondon United Kingdom"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0045-6535(97)00363-9"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.62.11.4206-4209.1996"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02815552"},{"key":"e_1_3_2_44_2","doi-asserted-by":"crossref","unstructured":"Meschede\nT.\nVogelsberger\nR.\nRemediation of a mercury contaminated site in Egypt\nContaminated soil '95.\nvan den Brink\nW. J.\nBosman\nR.\nArendt\nF.\n1995\n237\n238\nKluwer Academic Publishers\nDordrecht The Netherlands","DOI":"10.1007\/978-94-011-0415-9_54"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.60.6.1956-1961.1994"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/0038-0717(93)90040-I"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(80)90475-3"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0953-7562(09)81260-4"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1008324111558"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1021\/bp00034a002"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.65.9.3805-3809.1999"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00292574"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.65.10.4458-4463.1999"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1002\/jobm.3620330411"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.63.10.3919-3925.1997"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1997.tb10432.x"},{"key":"e_1_3_2_57_2","first-page":"1","article-title":"Fate of polynuclear aromatic compounds (PNAs) in soil-plant systems.","volume":"88","author":"Sims R. C.","year":"1983","unstructured":"Sims R. C. Overcash M. R. Fate of polynuclear aromatic compounds (PNAs) in soil-plant systems. Residue Rev. 88 1983 1 68","journal-title":"Residue Rev."},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.53.2.292-297.1987"},{"key":"e_1_3_2_59_2","unstructured":"Sutherland\nJ. B.\nRafii\nF.\nKhan\nA. A.\nCerniglia\nC. E.\nMechanism of polycyclic aromatic hydrocarbon degradation\nMicrobial transformation and degradation of toxic organic chemicals.\nYoung\nL. Y.\nCerniglia\nC. E.\n1995\n269\n306\nWiley-Liss\nNew York N.Y"},{"key":"e_1_3_2_60_2","doi-asserted-by":"publisher","DOI":"10.1099\/13500872-140-1-19"},{"key":"e_1_3_2_61_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.8.2280"},{"key":"e_1_3_2_62_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00170201"},{"key":"e_1_3_2_63_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00265826"},{"key":"e_1_3_2_64_2","doi-asserted-by":"publisher","DOI":"10.1016\/0269-7491(93)90206-4"},{"key":"e_1_3_2_65_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002530051070"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.66.6.2471-2478.2000","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.66.6.2471-2478.2000","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T10:10:33Z","timestamp":1645611033000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.66.6.2471-2478.2000"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,6]]},"references-count":64,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2000,6]]}},"alternative-id":["10.1128\/AEM.66.6.2471-2478.2000"],"URL":"https:\/\/doi.org\/10.1128\/aem.66.6.2471-2478.2000","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,6]]},"assertion":[{"value":"2000-01-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2000-04-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2000-06-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}