{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T15:18:49Z","timestamp":1760887129707},"reference-count":23,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2000,7,1]],"date-time":"2000-07-01T00:00:00Z","timestamp":962409600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2000,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            2,3-Dichloro-1-propanol is more chemically stable than its isomer, 1,3-dichloro-2-propanol, and is therefore more difficult to degrade. The isolation of bacteria capable of complete mineralization of 2,3-dichloro-1-propanol was successful only from enrichments at high pH. The bacteria thus isolated were found to be members of the \u03b1 division of the\n            <jats:italic>Proteobacteria<\/jats:italic>\n            in the\n            <jats:italic>Rhizobium<\/jats:italic>\n            subdivision, most likely\n            <jats:italic>Agrobacterium<\/jats:italic>\n            sp. They could utilize both dihaloalcohol substrates and 2-chloropropionic acid. The growth of these strains in the presence of 2,3-dichloro-1-propanol was strongly affected by the pH and buffer strength of the medium. Under certain conditions, a ladder of four active dehalogenase bands could be visualized from this strain in activity gels. The enzyme involved in the complete mineralization of 2,3-dichloro-1-propanol was shown to have a native molecular weight of 114,000 and consisted of four subunits of similar molecular weights.\n          <\/jats:p>","DOI":"10.1128\/aem.66.7.2882-2887.2000","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:56:57Z","timestamp":1027763817000},"page":"2882-2887","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":33,"title":["Isolation and Characterization of 2,3-Dichloro-1-Propanol-Degrading Rhizobia"],"prefix":"10.1128","volume":"66","author":[{"given":"Agus J.","family":"Effendi","sequence":"first","affiliation":[{"name":"Cardiff School of Biosciences, Cardiff University, Cardiff CF10 3TL, Wales, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven D.","family":"Greenaway","sequence":"additional","affiliation":[{"name":"Cardiff School of Biosciences, Cardiff University, Cardiff CF10 3TL, Wales, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brian N.","family":"Dancer","sequence":"additional","affiliation":[{"name":"Cardiff School of Biosciences, Cardiff University, Cardiff CF10 3TL, Wales, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00700463"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0141-0229(97)00254-8"},{"key":"e_1_3_2_4_2","unstructured":"Bull A. T. D. J. Hardman P. J. Sallis and B. M. Stubbs. October 1992. Dehalogenation of organohalogen-containing compounds. European patent 0 510 987 A1 and U.S. patent 5 470 742 (November 1995)."},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)57021-6"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1061\/(ASCE)0733-9372(1997)123:10(982)"},{"key":"e_1_3_2_7_2","first-page":"117","article-title":"Dehalogenase in soil bacteria","volume":"123","author":"Hardman D. J.","year":"1981","unstructured":"Hardman D. J. Slater J. H. (1981) Dehalogenase in soil bacteria.J. Gen. Microbiol.123:117\u2013128.","journal-title":"J. Gen. Microbiol."},{"key":"e_1_3_2_8_2","first-page":"S400","article-title":"Acute toxicity of dichloropropanols in mice: comparison between toxicities of two isomers","volume":"35","author":"Hirata M.","year":"1993","unstructured":"Hirata M. Tanaka A. Omura M. Inoue N. 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