{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T05:16:20Z","timestamp":1767676580650},"reference-count":40,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2000,8,1]],"date-time":"2000-08-01T00:00:00Z","timestamp":965088000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2000,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The genotypic heterogeneity of\n            <jats:italic>Streptococcus oralis<\/jats:italic>\n            isolated from the oral cavity was investigated using repetitive extragenic palindromic PCR. Unrelated subjects harbored unique genotypes, with numerous genotypes being isolated from an individual.\n            <jats:italic>S. oralis<\/jats:italic>\n            is the predominant aciduric bacterium isolated from noncarious tooth sites. Genotypic comparison of the aciduric populations isolated at pH 5.2 with those isolated from mitis-salivarius agar (MSA) (pH 7.0) indicated that the aciduric populations were genotypically distinct in the majority of subjects (\u03c7\n            <jats:sup>2<\/jats:sup>\n            = 13.09;\n            <jats:italic>P<\/jats:italic>\n            = 0.0031). Neither the aciduric nor the MSA-isolated strains were stable, with no strains isolated at baseline being isolated 4 or 12 weeks later in the majority of subjects. The basis of this instability is unknown but is similar to that reported for\n            <jats:italic>Streptococcus mitis<\/jats:italic>\n            . Examination of\n            <jats:italic>S. oralis<\/jats:italic>\n            strains isolated from cohabiting couples demonstrated that in three of five couples, genotypically identical strains were isolated from both partners and this was confirmed by using\n            <jats:italic>Salmonella enteritidis<\/jats:italic>\n            repetitive element PCR and enterobacterial PCR typing. These data provide further evidence of the physiological and genotypic heterogeneity of non-mutans streptococci. The demonstration of distinct aciduric populations of\n            <jats:italic>S. oralis<\/jats:italic>\n            implies that the role of these and other non-mutans streptococci in the caries process requires reevaluation.\n          <\/jats:p>","DOI":"10.1128\/aem.66.8.3330-3336.2000","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:55:30Z","timestamp":1027763730000},"page":"3330-3336","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":38,"title":["Genotypic Heterogeneity of\n            <i>Streptococcus oralis<\/i>\n            and Distinct Aciduric Subpopulations in Human Dental Plaque"],"prefix":"10.1128","volume":"66","author":[{"given":"S.","family":"Alam","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Dental Caries Research Group, Guy's, King's, and St. Thomas' Dental Institute, London SE5 9RW,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. R.","family":"Brailsford","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Dental Caries Research Group, Guy's, King's, and St. Thomas' Dental Institute, London SE5 9RW,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Adams","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Unilever Research, Port Sunlight, Wirral L63 3JW,2 England"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Allison","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Unilever Research, Port Sunlight, Wirral L63 3JW,2 England"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Sheehy","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Dental Caries Research Group, Guy's, King's, and St. Thomas' Dental Institute, London SE5 9RW,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Zoitopoulos","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Dental Caries Research Group, Guy's, King's, and St. Thomas' Dental Institute, London SE5 9RW,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E. 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