{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T12:49:09Z","timestamp":1791118149180,"version":"4.1.0"},"reference-count":32,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2000,8,1]],"date-time":"2000-08-01T00:00:00Z","timestamp":965088000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2000,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Restriction fragment length polymorphism and DNA sequence analysis discern two main types of\n            <jats:italic>Cryptosporidium parvum<\/jats:italic>\n            . We present a survey of length polymorphism at several microsatellite loci for type 1 and type 2 isolates. A total of 14 microsatellite loci were identified from\n            <jats:italic>C. parvum<\/jats:italic>\n            DNA sequences deposited in public databases. All repeats were mono-, di-, and trinucleotide repeats of A, AT, and AAT, reflecting the high AT content of the\n            <jats:italic>C. parvum<\/jats:italic>\n            genome. Several of these loci showed significant length polymorphism, with as many as seven alleles identified for a single locus. Differences between alleles ranged from 1 to 27 bp. Karyotype analysis using probes flanking three microsatellites localized each marker to an individual chromosomal band, suggesting that these markers are single copy. In a sample of 19 isolates for which at least three microsatellites were typed, a majority of isolates displayed a unique multilocus fingerprint. Microsatellite analysis of isolates passaged between different host species identified genotypic changes consistent with changes in parasite populations.\n          <\/jats:p>","DOI":"10.1128\/aem.66.8.3344-3349.2000","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:58:35Z","timestamp":1027763915000},"page":"3344-3349","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":54,"title":["Extensive Polymorphism in\n            <i>Cryptosporidium parvum<\/i>\n            Identified by Multilocus Microsatellite Analysis"],"prefix":"10.1128","volume":"66","author":[{"given":"Xiaochuan","family":"Feng","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Tufts University School of Veterinary Medicine, Division of Infectious Diseases, North Grafton, Massachusetts,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen M.","family":"Rich","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Tufts University School of Veterinary Medicine, Division of Infectious Diseases, North Grafton, Massachusetts,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donna","family":"Akiyoshi","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Tufts University School of Veterinary Medicine, Division of Infectious Diseases, North Grafton, Massachusetts,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James K.","family":"Tumwine","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Mulago Hospital, Makerere University, Kampala, Uganda2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Addy","family":"Kekitiinwa","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Mulago Hospital, Makerere University, Kampala, Uganda2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolette","family":"Nabukeera","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Mulago Hospital, Makerere University, Kampala, Uganda2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saul","family":"Tzipori","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Tufts University School of Veterinary Medicine, Division of Infectious Diseases, North Grafton, Massachusetts,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanni","family":"Widmer","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Tufts University School of Veterinary Medicine, Division of Infectious Diseases, North Grafton, Massachusetts,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"46S","article-title":"Microsatellite analysis of the human and bovine genotypes of Cryptosporidium parvum.","volume":"46","author":"Aiello A. 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Widmer. In F. Petri (ed.) The biology of Cryptosporidium in press. Cryptosporidosis and microsporidiosis. Karger Basel Switzerland."},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-308X(08)60122-0"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1086\/515373"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.64.11.4477-4481.1998"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(00)00211-5"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.66.8.3344-3349.2000","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.66.8.3344-3349.2000","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T10:21:55Z","timestamp":1645611715000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.66.8.3344-3349.2000"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,8]]},"references-count":32,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2000,8]]}},"alternative-id":["10.1128\/AEM.66.8.3344-3349.2000"],"URL":"https:\/\/doi.org\/10.1128\/aem.66.8.3344-3349.2000","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,8]]},"assertion":[{"value":"2000-03-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2000-05-17","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2000-08-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}