{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T06:06:45Z","timestamp":1791007605449,"version":"4.1.0"},"reference-count":20,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2000,9,1]],"date-time":"2000-09-01T00:00:00Z","timestamp":967766400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2000,9]]},"abstract":"ABSTRACT<\/jats:title>\n \n Of 100 strains of iron-oxidizing bacteria isolated,\n Thiobacillus ferrooxidans<\/jats:italic>\n SUG 2-2 was the most resistant to mercury toxicity and could grow in an Fe\n 2+<\/jats:sup>\n medium (pH 2.5) supplemented with 6 \u03bcM Hg\n 2+<\/jats:sup>\n . In contrast,\n T. ferrooxidans<\/jats:italic>\n AP19-3, a mercury-sensitive\n T. ferrooxidans<\/jats:italic>\n strain, could not grow with 0.7 \u03bcM Hg\n 2+<\/jats:sup>\n . When incubated for 3 h in a salt solution (pH 2.5) with 0.7 \u03bcM Hg\n 2+<\/jats:sup>\n , resting cells of resistant and sensitive strains volatilized approximately 20 and 1.7%, respectively, of the total mercury added. The amount of mercury volatilized by resistant cells, but not by sensitive cells, increased to 62% when Fe\n 2+<\/jats:sup>\n was added. The optimum pH and temperature for mercury volatilization activity were 2.3 and 30\u00b0C, respectively. Sodium cyanide, sodium molybdate, sodium tungstate, and silver nitrate strongly inhibited the Fe\n 2+<\/jats:sup>\n -dependent mercury volatilization activity of\n T. ferrooxidans<\/jats:italic>\n . When incubated in a salt solution (pH 3.8) with 0.7 \u03bcM Hg\n 2+<\/jats:sup>\n and 1 mM Fe\n 2+<\/jats:sup>\n , plasma membranes prepared from resistant cells volatilized 48% of the total mercury added after 5 days of incubation. However, the membrane did not have mercury reductase activity with NADPH as an electron donor. Fe\n 2+<\/jats:sup>\n -dependent mercury volatilization activity was not observed with plasma membranes pretreated with 2 mM sodium cyanide. Rusticyanin from resistant cells activated iron oxidation activity of the plasma membrane and activated the Fe\n 2+<\/jats:sup>\n -dependent mercury volatilization activity of the plasma membrane.\n <\/jats:p>","DOI":"10.1128\/aem.66.9.3823-3827.2000","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:55:30Z","timestamp":1027763730000},"page":"3823-3827","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":39,"title":["Ferrous Iron-Dependent Volatilization of Mercury by the Plasma Membrane of\n Thiobacillus ferrooxidans<\/i>"],"prefix":"10.1128","volume":"66","author":[{"given":"Kenji","family":"Iwahori","sequence":"first","affiliation":[{"name":"Science and Technology for Energy Conversion,1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fumiaki","family":"Takeuchi","sequence":"additional","affiliation":[{"name":"Administration Center for Environmental Science and Technology,2 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuo","family":"Kamimura","sequence":"additional","affiliation":[{"name":"Department of Biological Function, Faculty of Agriculture,3 Okayama University, Tsushima Naka, Okayama 700-8530, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsuyoshi","family":"Sugio","sequence":"additional","affiliation":[{"name":"Science and Technology for Energy Conversion,1"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1139\/m91-106"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)48126-4"},{"key":"e_1_3_1_4_2","first-page":"725","article-title":"The isolation of a mercuric ion-reducing flavoprotein from Thiobacillus ferrooxidans","volume":"130","author":"Booth J. 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