{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T00:05:23Z","timestamp":1790985923028,"version":"4.1.0"},"reference-count":32,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2001,10,1]],"date-time":"2001-10-01T00:00:00Z","timestamp":1001894400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2001,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The fate of salmonellae applied to tomato plants was investigated. Five\n            <jats:italic>Salmonella<\/jats:italic>\n            serotypes were used to inoculate tomato plants before and after fruits set, either by injecting stems with inoculum or brushing flowers with it. Ripe tomato fruits were subjected to microbiological analysis. Peptone wash water, homogenates of stem scar tissues, and homogenates of fruit pulp were serially diluted and plated on bismuth sulfite agar before and after enrichment. Presumptive\n            <jats:italic>Salmonella<\/jats:italic>\n            colonies were confirmed by serological tests, PCR assay using HILA2 primers, and enterobacterial repetitive intergenic consensus PCR. Of 30 tomatoes harvested from inoculated plants, 11 (37%) were positive for\n            <jats:italic>Salmonella<\/jats:italic>\n            . Of the\n            <jats:italic>Salmonella<\/jats:italic>\n            -positive tomatoes, 43 and 40%, respectively, were from plants receiving stem inoculation before and after flower set. Two of eight tomatoes produced from inoculated flowers contained\n            <jats:italic>Salmonella<\/jats:italic>\n            . Higher percentages of surface (82%) and stem scar tissue (73%) samples, compared to pulp of\n            <jats:italic>Salmonella<\/jats:italic>\n            -positive tomatoes (55%), harbored the pathogen. Of the five serotypes in the inoculum, Montevideo was the most persistent, being isolated from tomatoes 49 days after inoculation, and Poona was the most dominant, being present in 5 of 11\n            <jats:italic>Salmonella-<\/jats:italic>\n            positive tomatoes. Results suggest that\n            <jats:italic>Salmonella<\/jats:italic>\n            cells survive in or on tomato fruits from the time of inoculation at flowering through fruit ripening. Tomato stems and flowers are possible sites at which\n            <jats:italic>Salmonella<\/jats:italic>\n            may attach and remain viable during fruit development, thus serving as routes or reservoirs for contaminating ripened fruit.\n          <\/jats:p>","DOI":"10.1128\/aem.67.10.4760-4764.2001","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:00:58Z","timestamp":1027764058000},"page":"4760-4764","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":177,"title":["Survival of Salmonellae on and in Tomato Plants from the Time of Inoculation at Flowering and Early Stages of Fruit Development through Fruit Ripening"],"prefix":"10.1128","volume":"67","author":[{"given":"Xuan","family":"Guo","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Center for Food Safety and Department of Food Science and Technology, University of Georgia, Griffin, Georgia 30223-1797,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinru","family":"Chen","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Center for Food Safety and Department of Food Science and Technology, University of Georgia, Griffin, Georgia 30223-1797,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert E.","family":"Brackett","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Center for Food Safety and Department of Food Science and Technology, University of Georgia, Griffin, Georgia 30223-1797,1 and"},{"name":"<!--label omitted: 2-->Office of Plant, Dairy Foods and Beverages, U.S. Food and Drug Administration, Washington, D.C. 202042"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Larry R.","family":"Beuchat","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Center for Food Safety and Department of Food Science and Technology, University of Georgia, Griffin, Georgia 30223-1797,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","unstructured":"Agrios\nG. N.\nPlant pathology.\n1997\nAcademic Press\nSan Diego Calif"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0303.970304"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0168-1605(91)90059-X"},{"key":"e_1_3_2_5_2","unstructured":"Beuchat\nL. R.\nSurface decontamination of fruits and vegetables eaten raw: a review. W. H. O.\/FSF\/FOS\/98.2.\n1998\nWorld Health Organization\nGeneva Switzerland"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0304.970407"},{"key":"e_1_3_2_7_2","first-page":"29","article-title":"An outbreak of Salmonella javiana associated with consumption of watermelon.","volume":"56","author":"Blostein J.","year":"1991","unstructured":"Blostein J. An outbreak of Salmonella javiana associated with consumption of watermelon. J. Environ. Health 56 1991 29 31","journal-title":"J. Environ. Health"},{"key":"e_1_3_2_8_2","unstructured":"Bowes\nB. G.\nA color atlas of plant structure.\n1996\nManson Publishing Ltd.\nLondon United Kingdom"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-5214(98)00096-9"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-62.5.444"},{"key":"e_1_3_2_11_2","first-page":"87","article-title":"Salmonella typhimurium outbreak traced to a commercial apple cider\u2014New Jersey.","volume":"24","author":"Centers for Disease Control and Prevention","year":"1975","unstructured":"Centers for Disease Control and Prevention Salmonella typhimurium outbreak traced to a commercial apple cider\u2014New Jersey. Morbid. Mortal. Weekly Rep. 24 1975 87 88","journal-title":"Morbid. Mortal. Weekly Rep."},{"key":"e_1_3_2_12_2","first-page":"522","article-title":"Salmonella oranienburg gastroenteritis associated with consumption of precut watermelons, Illinois.","volume":"28","author":"Centers for Disease Control and Prevention","year":"1979","unstructured":"Centers for Disease Control and Prevention Salmonella oranienburg gastroenteritis associated with consumption of precut watermelons, Illinois. Morbid. Mortal. Weekly Rep. 28 1979 522 523","journal-title":"Morbid. Mortal. Weekly Rep."},{"key":"e_1_3_2_13_2","first-page":"549","article-title":"Multi-state outbreak of Salmonella poona infections\u2014United States and Canada.","volume":"40","author":"Centers for Disease Control and Prevention","year":"1991","unstructured":"Centers for Disease Control and Prevention Multi-state outbreak of Salmonella poona infections\u2014United States and Canada. Morbid. Mortal. Weekly Rep. 40 1991 549 552","journal-title":"Morbid. Mortal. Weekly Rep."},{"key":"e_1_3_2_14_2","unstructured":"Centers for Disease Control and Prevention\nMultistate outbreak of Salmonella serotype Montevideo infections. Publication EPI-AID 93\u201397.\n1993\nCenters for Disease Control and Prevention\nAtlanta Ga"},{"key":"e_1_3_2_15_2","first-page":"582","article-title":"Outbreak of Salmonella serotype Muenchen infections associated with unpasteurized orange juice\u2014United States and Canada.","volume":"48","author":"Centers for Disease Control and Prevention","year":"1999","unstructured":"Centers for Disease Control and Prevention Outbreak of Salmonella serotype Muenchen infections associated with unpasteurized orange juice\u2014United States and Canada. Morbid. Mortal. Weekly Rep. 48 1999 582 585","journal-title":"Morbid. Mortal. Weekly Rep."},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2621.1970.tb12176.x"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0956-7135(98)00022-X"},{"key":"e_1_3_2_18_2","first-page":"15","article-title":"Ricerche di microflora im pomodori sani.","volume":"41","author":"Ercolani G. L.","year":"1966","unstructured":"Ercolani G. L. Casolari A. Ricerche di microflora im pomodori sani. Ind. Conserve Parma 41 1966 15 22","journal-title":"Ind. Conserve Parma"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.2307\/4589055"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.12.5248-5252.2000"},{"key":"e_1_3_2_21_2","unstructured":"Hoagland\nD. R.\nArnon\nD. I.\nThe water-culture method for growing plants without soil. Circular 347. Agricultural Experiment Station\n1950\nUniversity of California\nBerkeley"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1991.tb00755.x"},{"key":"e_1_3_2_23_2","unstructured":"Jones\nJ. B.\nJr.\nTomato plant culture: in the field greenhouse and home garden.\n1998\nCRC Press\nBoca Raton Fla"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1086\/513985"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0505.990502"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1094\/Phyto-65-670"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268800059392"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2621.1963.tb00194.x"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-60.11.1400"},{"key":"e_1_3_2_30_2","unstructured":"United States Department of Agriculture\nAgricultural statistics 1997.\n1997\nUSDA National Agricultural Service. United States Government Printing Office\nWashington D.C."},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-58.8.829"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1605(00)00415-3"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.61.6.2127-2131.1995"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.67.10.4760-4764.2001","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.67.10.4760-4764.2001","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T10:01:07Z","timestamp":1645610467000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.67.10.4760-4764.2001"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,10]]},"references-count":32,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2001,10]]}},"alternative-id":["10.1128\/AEM.67.10.4760-4764.2001"],"URL":"https:\/\/doi.org\/10.1128\/aem.67.10.4760-4764.2001","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,10]]},"assertion":[{"value":"2001-03-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2001-07-16","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2001-10-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}