{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T19:44:50Z","timestamp":1790970290954,"version":"4.1.0"},"reference-count":68,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2001,10,1]],"date-time":"2001-10-01T00:00:00Z","timestamp":1001894400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2001,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Burkholderia<\/jats:italic>\n            sp. strain JS150 is able to metabolize a wide range of alkyl-and chloroaromatic hydrocarbons through multiple, apparently redundant catabolic pathways. Previous research has shown that strain JS150 is able to synthesize enzymes for multiple upper pathways as well as multiple lower pathways to accommodate variously substituted catechols that result from degradation of complex mixtures of monoaromatic compounds. We report here the genetic organization and functional characterization of a gene cluster, designated\n            <jats:italic>tbc<\/jats:italic>\n            (for toluene, benzene, and chlorobenzene utilization), which has been cloned as a 14.3-kb DNA fragment from strain JS150 into vector pRO1727. The cloned DNA fragment expressed in\n            <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n            PAO1c allowed the recombinant to grow on toluene or benzene and to transform chlorobenzene, trichloroethylene, phenol, and cresols. The\n            <jats:italic>tbc<\/jats:italic>\n            genes are organized into two divergently transcribed operons,\n            <jats:italic>tbc1<\/jats:italic>\n            and\n            <jats:italic>tbc2<\/jats:italic>\n            , each comprised of six open reading frames. Similarity searches of databases revealed that the\n            <jats:italic>tbc1<\/jats:italic>\n            and\n            <jats:italic>tbc2<\/jats:italic>\n            genes showed significant homology to multicomponent cresol and phenol hydroxylases and to toluene and benzene monooxygenases, respectively. Deletion mutagenesis and product analysis were used to demonstrate that\n            <jats:italic>tbc2<\/jats:italic>\n            plays a role in the initial catabolism of the unactivated alkyl- or chloroaromatic substrate and that the\n            <jats:italic>tbc1<\/jats:italic>\n            gene products play a role in the catabolism of the first metabolite that results from transformation of the initial substrate. Phylogenetic analysis was used to compare individual components of these\n            <jats:italic>tbc<\/jats:italic>\n            monooxygenases with similar sequences in the databases. These results provide further evidence for the existence of multiple, functionally redundant alkyl- and chloroaromatic monooxygenases in strain JS150.\n          <\/jats:p>","DOI":"10.1128\/aem.67.10.4805-4816.2001","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:00:58Z","timestamp":1027764058000},"page":"4805-4816","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":35,"title":["Genetic and Functional Analysis of the\n            <i>tbc<\/i>\n            Operons for Catabolism of Alkyl- and Chloroaromatic Compounds in\n            <i>Burkholderia<\/i>\n            sp. Strain JS150"],"prefix":"10.1128","volume":"67","author":[{"given":"Hyung-Yeel","family":"Kahng","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Biotechnology Center for Agriculture and the Environment1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana C.","family":"Malinverni","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Biotechnology Center for Agriculture and the Environment1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle M.","family":"Majko","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Biotechnology Center for Agriculture and the Environment1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jerome J.","family":"Kukor","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Biotechnology Center for Agriculture and the Environment1 and"},{"name":"<!--label omitted: 2-->Department of Environmental Sciences,2 Rutgers University, New Brunswick, New Jersey 08901-8520"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-144-10-2895"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.64.11.4353-4356.1998"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.64.10.3626-3632.1998"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(94)00844-I"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.178.21.6327-6337.1996"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1991.tb02114.x"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1030490104"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.169.6.2398-2404.1987"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1995.mmi_18010013.x"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00209a008"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.6.2486"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)38005-0"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00809a023"},{"key":"e_1_3_2_15_2","unstructured":"Gibson\nD. 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