{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T15:35:58Z","timestamp":1769873758947,"version":"3.49.0"},"reference-count":27,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2001,11,1]],"date-time":"2001-11-01T00:00:00Z","timestamp":1004572800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2001,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            In May 1999, field surveys of Lyme disease spirochetes were conducted around the Tianshan Mountains in Xinjiang Uygur Autonomous Region in northwestern People's Republic of China.\n            <jats:italic>Ixodes persulcatus<\/jats:italic>\n            ticks were obtained in a Tianchi Lake valley with primary forest, while the tick fauna was poor in the semidesert or at higher altitudes in this region. Species identities were confirmed by molecular analysis in which an internal transcribed spacer sequence was used. Of 55 adult ticks, 22 (40%) were positive for spirochetes as determined by Barbour-Stoenner-Kelly culture passages. In addition, some rodents, including\n            <jats:italic>Apodemus uralensis<\/jats:italic>\n            (5 of 14 animals) and\n            <jats:italic>Cricetulus longicaudatus<\/jats:italic>\n            (the only animal examined), and some immature stages of\n            <jats:italic>I. persulcatus<\/jats:italic>\n            (4 of 11 ticks) that had fed on\n            <jats:italic>A. uralensis<\/jats:italic>\n            were positive for spirochetes. Based on 5S-23S rRNA intergenic spacer restriction fragment length polymorphism analysis and reactivity with monoclonal antibodies, 35 cultures (including double isolation cultures) were identified as\n            <jats:italic>Borrelia garinii<\/jats:italic>\n            (20 isolates, including 9 Eurasian pattern B isolates and 11 Asian pattern C isolates),\n            <jats:italic>Borrelia afzelii<\/jats:italic>\n            (10 pattern D isolates), and mixed cultures (5 cultures, including isolates that produced\n            <jats:italic>B. garinii<\/jats:italic>\n            patterns B and C plus\n            <jats:italic>B. afzelii<\/jats:italic>\n            pattern D). These findings revealed that Lyme disease pathogens are distributed in the mountainous areas in northwestern China even though it is an arid region, and they also confirmed the specific relationship between\n            <jats:italic>I. persulcatus<\/jats:italic>\n            and genetic patterns of\n            <jats:italic>Borrelia<\/jats:italic>\n            spp. on the Asian continent.\n          <\/jats:p>","DOI":"10.1128\/aem.67.11.5161-5165.2001","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:03:59Z","timestamp":1027764239000},"page":"5161-5165","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":29,"title":["Lyme Disease\n            <i>Borrelia<\/i>\n            spp. in Ticks and Rodents from Northwestern China"],"prefix":"10.1128","volume":"67","author":[{"given":"Nobuhiro","family":"Takada","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Immunology and Medical Zoology, Fukui Medical University, Matsuoka, Fukui 910-1193,1"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshiyuki","family":"Masuzawa","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Department of Microbiology, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka 422-8526,2"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fubito","family":"Ishiguro","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->Fukui Prefectural Institute of Public Health, Fukui 910-8551,3"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiromi","family":"Fujita","sequence":"additional","affiliation":[{"name":"<!--label omitted: 4-->Research Laboratory of Ohara General Hospital, Fukushima 960-0195,4"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Midori","family":"Kudeken","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Department of Microbiology, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka 422-8526,2"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harumi","family":"Mitani","sequence":"additional","affiliation":[{"name":"<!--label omitted: 5-->Laboratory of Molecular Microbiology, Faculty of Pharmacy and Pharmaceutical Science, Fukuyama University, Fukuyama 729-0292,5 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahito","family":"Fukunaga","sequence":"additional","affiliation":[{"name":"<!--label omitted: 5-->Laboratory of Molecular Microbiology, Faculty of Pharmacy and Pharmaceutical Science, Fukuyama University, Fukuyama 729-0292,5 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kimiyuki","family":"Tsuchiya","sequence":"additional","affiliation":[{"name":"<!--label omitted: 6-->Experimental Animal Center, Miyazaki Medical College, Kiyotake 889-1692,6 Japan; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuhiro","family":"Yano","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Immunology and Medical Zoology, Fukui Medical University, Matsuoka, Fukui 910-1193,1"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao-Hang","family":"Ma","sequence":"additional","affiliation":[{"name":"<!--label omitted: 7-->Department of Microbiology, Faculty of Life Science, Zhejiang University, Hangzhou, People's Republic of China7"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/ije\/19.4.1061"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-42-3-378"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01566969"},{"key":"e_1_3_2_5_2","unstructured":"Editorial Committee of the Distribution Mammalian Species in China\nDistribution of mammalian species in China\n1997\n251\nChina Forestry Publishing House\nBeijing People's Republic of China(In Chinese.)"},{"key":"e_1_3_2_6_2","unstructured":"Filippova\nN. A.\nTaiga tick Ixodes persulcatus (Acarina: Ixodidae). Morphplogy systematics ecology medical importance\n1985\n416\nSoviet Committee for the UNESCO Programme Man and Biosphere\nLeningrad Russia (In Russian.)"},{"key":"e_1_3_2_7_2","unstructured":"Filippova N. A. 1999. Systematic relationships of the Ixodes ricinus complex in the palearctic faunal region p. 355\u2013361. In G. R. Needham R. Mitchell D. J. Horn and W. C. Welbourn (ed.) Acarology IX vol. 2. Symposium. Ohio Biological SurveyColumbus Ohio."},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1645\/0022-3395(2000)086[0038:MPAOIT]2.0.CO;2"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.3.982-986.2000"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.64.7.2705-2709.1998"},{"key":"e_1_3_2_11_2","unstructured":"Ma\nY.\nWang\nF.\nJin\nS.\nLi\nS.\nGlires (rodents and lagomorphs) of northern Xinjiang and their zoogeographical distribution\n1987\n274\nScience Press of Academia Sinica\nBeijing People's Republic of China (In Chinese.)"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.1997.tb01897.x"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.1996.tb01093.x"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1996.tb08411.x"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.29.8.1568-1573.1991"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.1996.tb01106.x"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/170.4.878"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-44-4-743"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.62.10.3887-3889.1996"},{"key":"e_1_3_2_20_2","unstructured":"Takada\nN.\nA pictorial review of medical acarology in Japan\n1990\n216\nKinpodo\nKyoto Japan (In Japanese.)"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.2307\/3284712"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/jmedent\/31.3.474"},{"key":"e_1_3_2_23_2","unstructured":"Teng\nK. 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Ser."},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.1997.tb01053.x"},{"key":"e_1_3_2_28_2","unstructured":"Zhang\nZ.\nEpidemiological features of Lyme disease in China\nProceedings of the International Symposium on Lyme Disease in Japan.\nYanagihara\nY.\nMasuzawa\nT.\n1994\n84\n91\nUniversity of Shizuoka\nShizuoka Japan"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.67.11.5161-5165.2001","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.67.11.5161-5165.2001","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T10:15:37Z","timestamp":1645611337000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.67.11.5161-5165.2001"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,11]]},"references-count":27,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2001,11]]}},"alternative-id":["10.1128\/AEM.67.11.5161-5165.2001"],"URL":"https:\/\/doi.org\/10.1128\/aem.67.11.5161-5165.2001","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,11]]},"assertion":[{"value":"2001-04-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2001-08-03","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2001-11-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}