{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T12:43:10Z","timestamp":1772196190282,"version":"3.50.1"},"reference-count":50,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2001,12,1]],"date-time":"2001-12-01T00:00:00Z","timestamp":1007164800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2001,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A Tn\n            <jats:italic>5<\/jats:italic>\n            -based mutagenesis strategy was used to generate a collection of trichloroethylene (TCE)-sensitive (TCS) mutants in order to identify repair systems or protective mechanisms that shield\n            <jats:italic>Burkholderia cepacia<\/jats:italic>\n            G4 from the toxic effects associated with TCE oxidation. Single Tn\n            <jats:italic>5<\/jats:italic>\n            insertion sites were mapped within open reading frames putatively encoding enzymes involved in DNA repair (UvrB, RuvB, RecA, and RecG) in 7 of the 11 TCS strains obtained (4 of the TCS strains had a single Tn\n            <jats:italic>5<\/jats:italic>\n            insertion within a\n            <jats:italic>uvrB<\/jats:italic>\n            homolog). The data revealed that the\n            <jats:italic>uvrB<\/jats:italic>\n            -disrupted strains were exceptionally susceptible to killing by TCE oxidation, followed by the\n            <jats:italic>recA<\/jats:italic>\n            strain, while the\n            <jats:italic>ruvB<\/jats:italic>\n            and\n            <jats:italic>recG<\/jats:italic>\n            strains were just slightly more sensitive to TCE than the wild type. The\n            <jats:italic>uvrB<\/jats:italic>\n            and\n            <jats:italic>recA<\/jats:italic>\n            strains were also extremely sensitive to UV light and, to a lesser extent, to exposure to mitomycin C and H\n            <jats:sub>2<\/jats:sub>\n            O\n            <jats:sub>2<\/jats:sub>\n            . The data from this study establishes that there is a link between DNA repair and the ability of\n            <jats:italic>B. cepacia<\/jats:italic>\n            G4 cells to survive following TCE transformation. A possible role for nucleotide excision repair and recombination repair activities in TCE-damaged cells is discussed.\n          <\/jats:p>","DOI":"10.1128\/aem.67.12.5384-5391.2001","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:03:59Z","timestamp":1027764239000},"page":"5384-5391","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":18,"title":["Requirement of DNA Repair Mechanisms for Survival of\n            <i>Burkholderia cepacia<\/i>\n            G4 upon Degradation of Trichloroethylene"],"prefix":"10.1128","volume":"67","author":[{"given":"Chris M.","family":"Yeager","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Molecular and Cellular Biology Program,1"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter J.","family":"Bottomley","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Molecular and Cellular Biology Program,1"},{"name":"<!--label omitted: 2-->Department of Microbiology and Crop and Soil Sciences,2 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel J.","family":"Arp","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Molecular and Cellular Biology Program,1"},{"name":"<!--label omitted: 3-->Department of Botany and Plant Pathology,3 Oregon State University, Corvallis, Oregon 97331-2902"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0958-1669(95)80059-X"},{"key":"e_1_3_2_3_2","first-page":"776","article-title":"Covalent binding of the carcinogen trichloroethylene to hepatic microsomal proteins and to exogenous DNA in vitro.","volume":"38","author":"Banerjee S.","year":"1978","unstructured":"Banerjee S. 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