{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T14:20:25Z","timestamp":1770214825643,"version":"3.49.0"},"reference-count":19,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2001,12,1]],"date-time":"2001-12-01T00:00:00Z","timestamp":1007164800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2001,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The application of genotyping to clinical isolates of<jats:italic>Cryptosporidium<\/jats:italic>has increased significantly our knowledge and understanding of the distribution and epidemiology of this parasite. However, some methods can be laborious and demand specialist technical expertise. PCR-restriction fragment length polymorphism (RFLP) techniques represent a more rapid and simple method of genotyping to support epidemiological and clinical investigations than conventional DNA analytical techniques. We describe a nested PCR-RFLP technique that identifies polymorphisms in the<jats:italic>C<\/jats:italic>.<jats:italic>parvum<\/jats:italic>thrombospondin-related adhesive protein gene locus; this method offers a sensitive and specific tool for the confirmation and investigation of disease associated with<jats:italic>C<\/jats:italic>.<jats:italic>parvum<\/jats:italic>. The potential of this enhanced method is demonstrated by its application to the confirmation and epidemiological investigation of an outbreak of cryptosporidiosis associated with a school visit to an open farm.<\/jats:p>","DOI":"10.1128\/aem.67.12.5581-5584.2001","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:00:58Z","timestamp":1027764058000},"page":"5581-5584","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":61,"title":["Modification of a Rapid Method for the Identification of Gene-Specific Polymorphisms in<i>Cryptosporidium<\/i><i>parvum<\/i>and Its Application to Clinical and Epidemiological Investigations"],"prefix":"10.1128","volume":"67","author":[{"given":"K.","family":"Elwin","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->PHLS Cryptosporidium Reference Unit, Swansea Public Health Laboratory, Singleton Hospital, Swansea, United Kingdom SA2 8QA1;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. 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